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Development and multiplexing of microsatellite loci for the near threatened freshwater mussel Potomida littoralis (Cuvier, 1798) using 454 sequencing

Authors

  • E. Froufe,

    Corresponding author
    • Centre of Marine and Environmental Research, Porto, Portugal
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  • C. Sobral,

    1. Forestry Department, Centre for Research and Technology of Agro-Environment and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • A. Teixeira,

    1. Mountain Research Centre, School of Agriculture, Polytechnic Institute of Bragança, Bragança, Portugal
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  • A. Lopes,

    1. Centre of Marine and Environmental Research, Porto, Portugal
    2. Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
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  • R. Sousa,

    1. Centre of Marine and Environmental Research, Porto, Portugal
    2. Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Braga, Portugal
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  • S. Varandas,

    1. Forestry Department, Centre for Research and Technology of Agro-Environment and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • M. Lopes-Lima

    1. Centre of Marine and Environmental Research, Porto, Portugal
    2. Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
    3. Zoomarine, Albufeira, Portugal
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Correspondence to: Elsa Froufe, Laboratory of Marine Community Ecology and Evolution, CIMAR-LA/CIIMAR-UP Centre of Marine and Environmental Research, Rua dos Bragas 289, 4050–123 Porto, Portugal. E-mail: elsafroufe@gmail.com

ABSTRACT

  1. The Unionidae are among the most endangered fauna in the world and globally in decline. They are particularly vulnerable to habitat loss and fragmentation, susceptible to flow, pollution and climatic disturbances and introduction of invasive species. Despite their well-recognized ecological and conservation importance, there is a surprising lack of genomic resources currently available for European species.
  2. The aim of this study was to develop and characterize microsatellites for the near threatened freshwater mussel, Potomida littoralis using 454 sequencing.
  3. In order to improve genotyping throughput as well as cost-effectiveness, two multiplex-PCR reactions were designed to amplify 16 new loci. All the new 16 microsatellites were successfully combined in two multiplexed PCR with the number of alleles ranging from 2 to 25 per locus (with a mean of 10), confirming the utility of the new markers.
  4. The new genetic markers can therefore be used for studying the population genetic structure and evolution of this species, e.g. to examine current levels of genetic variability within and between populations and thus to contribute to conservation and management. Copyright © 2013 John Wiley & Sons, Ltd.
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