S35 — liquid — scintillation count analysis of morphogenesis and teratogenesis of the palate in mouse embryos
Article first published online: 3 FEB 2005
Copyright © 1964 Wiley-Liss, Inc.
The Anatomical Record
Volume 150, Issue 3, pages 271–277, November 1964
How to Cite
Jacobs, R. M. (1964), S35 — liquid — scintillation count analysis of morphogenesis and teratogenesis of the palate in mouse embryos. Anat. Rec., 150: 271–277. doi: 10.1002/ar.1091500309
- Issue published online: 3 FEB 2005
- Article first published online: 3 FEB 2005
S35-labeled inorganic sulfate was employed as a precursor of sulfated mucopolysaccharides in a radioassay of embryonic palatine shelves of the offspring of normal and cortisone-treated mice. Samples, composed of four palatine shelves derived from the same litter, were counted on a liquid scintillation spectrometer. A close correlation has been established between the time of the maximum aggregation of sulfated mucopolysaccharides, as expressed by the relative specific activity of retained S35-labeled sulfate, and the time of “horizontalization” of palatine shelves in both experimental groups.
The competence of embryological mechanism of palate closure has been related to the developmentally normal relative increase of the concentration of sulfated mucopolysaccharides, viewed as a factor maintaining maximum visco-elasticity and minimal swelling of palatine shelves.