S35 — liquid — scintillation count analysis of morphogenesis and teratogenesis of the palate in mouse embryos


  • Richard M. Jacobs

    1. Department of Anatomy, Medical College of Virginia, Richmond, Virginia
    Current affiliation:
    1. Department of Orthodontics, College of Dentistry, University of Illinois, Chicago, Illinois 60612
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S35-labeled inorganic sulfate was employed as a precursor of sulfated mucopolysaccharides in a radioassay of embryonic palatine shelves of the offspring of normal and cortisone-treated mice. Samples, composed of four palatine shelves derived from the same litter, were counted on a liquid scintillation spectrometer. A close correlation has been established between the time of the maximum aggregation of sulfated mucopolysaccharides, as expressed by the relative specific activity of retained S35-labeled sulfate, and the time of “horizontalization” of palatine shelves in both experimental groups.

The competence of embryological mechanism of palate closure has been related to the developmentally normal relative increase of the concentration of sulfated mucopolysaccharides, viewed as a factor maintaining maximum visco-elasticity and minimal swelling of palatine shelves.