This paper was prepared (in part) from data in a dissertation submitted to Boston University Graduate School in partial fulfillment of the requirements for the Doctor of Philosophy degree.
Postnatal development of beta cells and ILA secretion in the pancreatic islets of the golden hamster†
Article first published online: 2 FEB 2005
Copyright © 1965 Wiley-Liss, Inc.
The Anatomical Record
Volume 152, Issue 1, pages 25–33, May 1965
How to Cite
Sak, M. F., Macchi, I. A. and Beaser, S. B. (1965), Postnatal development of beta cells and ILA secretion in the pancreatic islets of the golden hamster. Anat. Rec., 152: 25–33. doi: 10.1002/ar.1091520105
This investigation was supported (in part) by Research Grant AM-06873 from the National Institute for Arthritis and Metabolic Diseases, National Institutes of Health, USPHS, and the Green Shoe-Stride-Rite Charitable Foundation, Inc., Boston, Massachusetts.
- Issue published online: 2 FEB 2005
- Article first published online: 2 FEB 2005
Fetal and neonatal endocrine pancreas of the golden hamster (Mesocricetus auratus) was evaluated cytochemically with regard to maturation of beta cell granulation (aldehyde fuchsin technique) and the structural findings were related to the release of insulin-like activity (ILA) in vitro (rat epididymal fat pad bioassay).
In the fetal pancreas 12 hours before birth, beta cells could not be distinguished histochemically although considerable ILA was released. In the absence of distinct islands of Langerhans at birth, a few cells showed AF+ granulation which was accompanied by a slight increase in the ILA. Maximal release of ILA occurred approximately on the second postnatal day when the AF+ granulation was denser and more abundant than at earlier periods. Beyond the second day, the rate of in vitro ILA secretion decreased although islets and beta cells showed progressive structural maturation. There was also a relative increase in the amount of exocrine tissue in the portions of pancreas used for bioassay. Advancing proteolytic inactivation may have accounted for the decrease in effective ILA when the latter was expressed per unit weight of tissue.
The data indicate that in the golden hamster, beta cells are biochemically competent before they demonstrate cytochemical maturity.