Fine structural observations of the absorption of lipid particles in the small intestine of the rat

Authors

  • RøRgen Rostgaard,

    1. Department of Anatomy, Yale University School of Medicine, New Haven 11, Connecticut
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    • Anna Fuller Postdoctoral Fellow. Department of Anatomy, Royal Medical College, Copenhagen, Denmark.

  • Russell J. Barrnett

    1. Department of Anatomy, Yale University School of Medicine, New Haven 11, Connecticut
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  • This work was supported by grants from the National Institutes of Arthritis and Metabolic Diseases (AM 03688) and from the National Cancer Institute (5T1-CA-5055), National Institutes of Health, Department of Health, Education and Welfare.

Abstract

Globules or particles of material, 50 Å to 1000 Å in diameter, stained with Pb++ to become dense, occurred between, on, or within the microvilli of the absorptive cells of the proximal jejunum of rats which had been fasted for 48 hours and fed corn oil one hour before sacrifice. This material was demonstrable in tissues fixed in glutaraldehyde, osmium tetroxide, or both fixatives providing Pb++ staining occurred. No or very little stainable material was present in fasted rats. These findings were correlated with in vitro studies on negatively stained or Pb++ stained solutions of micelles. The absorption of particulate lipid by the microvilli of the epithelial cells of the small intestine is discussed.

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