Collection of mouse semen by electroejaculation


  • Supported in part by grant HD-00543 from the National Institutes of Health, Public Health Service. Technical assistance by Robert M. Callahan.


Liquid and coagulated ejaculates were collected separately from mice by electroejaculation. Sperm concentrations in the liquid ejaculates from 51 animals ranged between 25,000 and 2,228,000/mm3 and total number of spermatozoa per ejaculate between 60,000 and 5,464,000. This method is suggested as a practical means of obtaining ejaculated spermatozoa free of coagulum for reproduction research.