Male and female Taconic Swiss mice were fed a high-fat (28%), low-protein (8%), hypolipotropic diet. Intact mice and gonadectomized mice, with and without injections of estrone or testosterone phenylacetate were studied.

The sex of the animals did not alter the incidence or type of diet-induced cardiovascular lesions. During the 6–12 weeks of feeding thrombi developed in the atrial lumina of 75%–88% of intact and gonadectomized male and female mice. The only prominent decrease in the incidence of such thrombosis was in gonadectomized animals of both sexes receiving estrone where it was reduced to 6%–16%. In castrated males receiving injections of testosterone phenylacetate (Perandren) the incidence of thrombosis was 65%.