This work was supported in part by grant PHS NS 07472 from the National Institutes of Health.
Fine structure of degeneration and regeneration in denervated rabbit vallate taste buds†
Article first published online: 27 JAN 2005
Copyright © 1970 Wiley-Liss, Inc.
The Anatomical Record
Volume 168, Issue 3, pages 393–413, November 1970
How to Cite
Fujimoto, S. and Murray, R. G. (1970), Fine structure of degeneration and regeneration in denervated rabbit vallate taste buds. Anat. Rec., 168: 393–413. doi: 10.1002/ar.1091680306
- Issue published online: 27 JAN 2005
- Article first published online: 27 JAN 2005
- Manuscript Accepted: 29 MAY 1970
- Manuscript Received: 22 APR 1970
Taste buds of rabbit circumvallate papillae were studied with the electron microscope at intervals from six hours to 11 weeks after section of the glossopharyngeal nerve distal to the petrosal ganglion. Nerve endings were first affected, showing degeneration as early as 12 hours and disappearing by 48 hours. Rapid loss of cells and of all buds by ten days followed. Numerous inclusion bodies within type I and type II cells were interpreted as autophagic activity in type II cells and both phagocytic and autophagic activity in type I cells. Type III cells were lost primarily by pyknosis, and phagocytized by type I cells. No clear evidence of dedifferentiation, or extrusion of dead cells, was observed.
Regenerated nerves appeared beneath the epithelium at 21 days but new buds first appeared at 25 days, after nerves had penetrated the basement membrane. Intimate contact of nerves with epithelial cells appears to be a precondition for taste bud renewal. Early appearance of cells resembling basal cells (type IV) followed by relatively simultaneous appearance of type I, II and III suggest independent origins for these three types. The data support a humoral hypothesis of trophic action but do not rule out a role for impulse transmission.