Cell replication of mesenchymal elements in adult tissues. I. The replication and migration of mesenchymal cells in the adult rabbit dermis

Authors

  • Dr. Gordon I. Kaye,

    1. The F. Higginson Cabot Laboratory of the Division of Surgical Pathology, Departments of Surgery and Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Search for more papers by this author
    • Dr. Kaye is recipient of a Career Scientist Award of the Health Research Council of the City of New York, under Contract I-320.

  • Linda F. Siegel,

    1. The F. Higginson Cabot Laboratory of the Division of Surgical Pathology, Departments of Surgery and Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Search for more papers by this author
  • Robert R. Pascal

    1. The F. Higginson Cabot Laboratory of the Division of Surgical Pathology, Departments of Surgery and Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Search for more papers by this author

  • Supported in part by grant AM-12396 of the NIAMD, NIH, US PHS, by grant P-362 of the American Cancer Society, and by a gift from Mr. Jacques Weber.

Abstract

Autoradiographic determination of the number and distribution of replicating mesenchymal cells in rabbit dermis was carried out using animals sacrificed between two hours and 21 days after a multiple-dose-pulse of 3H-thymidine. Initial labeling of mesenchymal cells occurred principally in a zone immediately subjacent to the germinative epithelium. This was confirmed by in vitro studies of the uptake of 3H-Tdr by rabbit skin during one hour of incubation. Five days after the in vitro pulse of 3H-Tdr there was a fifteen-fold increase in the number of labeled cells, considerable dilution of label and migration of labeled cells so that they were evenly distributed throughout the full thickness of the dermis. At 21 days the number of labeled cells was similar to that found at five days, but there was further migration of labeled cells toward the deep dermis.

This tissue provides an example of appositional replication and divergent migration of an epithelial-mesenchymal system.

Ancillary