This research was supported by grant MA-3556 from the Medical Research Council of Canada.
Effect of sodium fluoride and sodium pyruvate on palatal development in vitro†
Article first published online: 27 JAN 2005
Copyright © 1971 Wiley-Liss, Inc.
The Anatomical Record
Volume 171, Issue 1, pages 39–52, September 1971
How to Cite
Myers, G. S. (1971), Effect of sodium fluoride and sodium pyruvate on palatal development in vitro. Anat. Rec., 171: 39–52. doi: 10.1002/ar.1091710104
- Issue published online: 27 JAN 2005
- Article first published online: 27 JAN 2005
- Manuscript Accepted: 12 FEB 1971
- Manuscript Received: 4 NOV 1970
Explants of the embryonic rat palate have been treated by adding sodium fluoride and sodium pyruvate to the culture medium. Fluoride, at specific concentrations, causes retardation of palatal shelf growth so that fusion does not occur during the culture period. Partial or complete fusion does occur if fluoride levels are reduced.
Sodium pyruvate added to the medium advances the time of fusion of explants over that found in controls. When combined with fluoride in the medium, pyruvate can reverse the effects of fluoride on shelf growth and permit complete fusion to take place in a laige percentage of explants.
The mode of action of flucride or pyruvate under these experimental conditions has not been determined. However, the known effects of fluoride as an enzyme inhibitor must be considered.