Part of this study was presented at the 9th International Congress on Electron Microscopy (Toronto, 1978)
Crystalloids of actin-like filaments in the sertoli cell of the swine testis†
Article first published online: 8 FEB 2005
Copyright © 1979 Wiley-Liss, Inc.
The Anatomical Record
Volume 195, Issue 1, pages 47–61, September 1979
How to Cite
Toyama, Y., Obinata, T. and Holtzer, H. (1979), Crystalloids of actin-like filaments in the sertoli cell of the swine testis. Anat. Rec., 195: 47–61. doi: 10.1002/ar.1091950105
- Issue published online: 8 FEB 2005
- Article first published online: 8 FEB 2005
- Manuscript Accepted: 5 MAR 1979
- Manuscript Received: 31 OCT 1978
Normal swine testes, congenital cryptorchid swine testes, and normal human testes were exposed to HMM (heavy meromyosin) after either glycerination or saponin treatment in order to determine whether the fine filaments composing the crystalloids in the Sertoli cells are actin-like. The microfilaments of the crystalloids in the Sertoli cells of the cryptorchid swine testes bind HMM to form arrowhead complexes. Short bundles of microfilaments observed in the basal part of the Sertoli cells in both normal and cryptorchid testes also bind HMM. Similar bundles of HMM-bound filaments are observed in the vicinity of spermatocytes. The periodicity of the arrowhead complexes is about 35 nm, and all arrowheads on a given filament point in the same direction. In addition, the polarity of the HMM-bound filaments in a given crystalloid or bundle is uni-directional. A mechanism for the formation of the swine crystalloids has been suggested by Toyama (′75), and the results of this study strongly support this hypothesis.
Fine filaments of Charcot-Boettcher's crystalloid in human Sertoli cells did not bind HMM. Therefore the fine filaments of the human crystalloid are not actin-like in nature.