Light and electron microscopic immunohistochemical localization of somatostatin in the endocrine and exocrine portions of the pancreas of the chick embryo (gallus domesticus)
Article first published online: 8 FEB 2005
Copyright © 1982 Wiley-Liss, Inc.
The Anatomical Record
Volume 202, Issue 3, pages 349–357, March 1982
How to Cite
Kalliecharan, R. and Steeves, J. (1982), Light and electron microscopic immunohistochemical localization of somatostatin in the endocrine and exocrine portions of the pancreas of the chick embryo (gallus domesticus). Anat. Rec., 202: 349–357. doi: 10.1002/ar.1092020307
- Issue published online: 8 FEB 2005
- Article first published online: 8 FEB 2005
- Manuscript Accepted: 15 SEP 1981
- Manuscript Received: 6 OCT 1980
A discrete population of cells containing immunoreactive somatostatin was shown by the peroxidase antibody bridge technique at both the light and electron microscopic level to be present in the pancreas of chick embryos. Based on the stage of development, somatostatin-positive cells, at the light microscopic level, were found in the alpha and beta islets, in isolated islets that did not correspond with any known alpha or beta islets, as well as in the acinar tissue. Quantitative determination indicated that, at all ages examined, there were a greater number of somotostatin cells associated with the alpha islets per square millimeter of tissue than with either the beta islets or acinar tissue of the exocrine pancreas.
Ultrastructural observations on day 15 of development confirm and reinforce the light microscopic observations pertaining to the localization of the somatostatin-positive cells. The results also show that the PAP reaction was localized only on the granules of the somatostatin positive cells. These granules were primarily found in the apical region of these cells adjacent to blood vessels.