Light-microscopic and microspectrofluorometric characterization of porphyrin-containing astrocytes in mouse optic nerve
Article first published online: 8 FEB 2005
Copyright © 1983 Wiley-Liss, Inc.
The Anatomical Record
Volume 205, Issue 3, pages 355–361, March 1983
How to Cite
Terr, L. I. and Weiner, L. P. (1983), Light-microscopic and microspectrofluorometric characterization of porphyrin-containing astrocytes in mouse optic nerve. Anat. Rec., 205: 355–361. doi: 10.1002/ar.1092050312
- Issue published online: 8 FEB 2005
- Article first published online: 8 FEB 2005
- Manuscript Accepted: 28 SEP 1982
- Manuscript Received: 15 JUL 1982
Fluorescence spectroscopy has been employed to investigate the emission spectrum of unusual orange-red fluorescence found in the mouse optic nerve. Comparison of the spectra obtained with those of a number of porphyrins used as standards (protoporphyrin, uroporphyrin, and coproporphyrins) shows that the autofluorescence excited at about 400 nm (Soret band) is due to the presence of a mixture of these or other porphyrins in the nerve. Phase contrast, dark-field, and light-microscopy techniques demonstrated that the fluorescence is emitted by dense, coarse inclusions in the cytoplasm of astrocytes. The inclusions also exhibit high activity of endogenous peroxidase, a heme (porphyrin)- containing enzyme, characteristic for process of phagocytosis. A possible particiption of these astrocytes in phagocytosis is delineated.