Gross morphological analysis of limb regeneration in postmetamorphic adult Ambystoma
Article first published online: 26 JAN 2005
Copyright © 1983 Wiley-Liss, Inc.
The Anatomical Record
Volume 206, Issue 3, pages 295–306, July 1983
How to Cite
Young, H. E., Bailey, C. F. and Dalley, B. K. (1983), Gross morphological analysis of limb regeneration in postmetamorphic adult Ambystoma. Anat. Rec., 206: 295–306. doi: 10.1002/ar.1092060308
- Issue published online: 26 JAN 2005
- Article first published online: 26 JAN 2005
- Manuscript Accepted: 22 MAR 1983
- Manuscript Received: 7 JUL 1982
Due to the great disparity between regeneration times for the larval salamander (40 days), axolotl (30+ days), newt (44 days), and adult salamander (155 to 370 days), a staging system was devised so correlative comparisons could be made between regenerative model systems. The sequence was based on two criteria: (1) the stages should be similar to previously reported sequences for the newt, axolotl, and larval salamander, and (2) the stages must be readily recognizable by examination of the external morphology in the living state. Postmetamorphic adult land-phase Ambystoma were amputated through the forearm, placed within survival conditions, and observed until regeneration was completed. Of the initial 160 salamanders, 157 (98%) progressed through 11 clearly defined stages of regeneration. A side-by-side comparison of the staging sequence for land and aquatic phase urodeles is given along with a summary of key external morphological characteristics for the adult salamander. It was noted that as the length of time for regeneration decreased, the relative ratio of the nerves innervating the limb (spinal nerves 4, 5, and 6) increased for the four species of Ambystoma examined: A. annulatum, 324 to 370 days postamputation (dpa) with a 1:1:1 neural tissue ratio; A. maculatum, 255 to 300 dpa with a 2:2:1 ratio; A. texanum, 215 to 250 dpa with a 2:2:2 ratio; and A. tigranum, 155 to 180 dpa with a 2:3:3 ratio.