Effects of two glycosaminoglycans on seeding of cardiac cushion tissue cells into a collagen-lattice culture system

Authors

  • Dr. David H. Bernanke,

    Corresponding author
    1. Department of Anatomy, Texas Tech University Health Sciences Center, Lubbock, TX 79430
    Current affiliation:
    1. Wound Healing Laboratory, Shriners Burns Institute, 51 Blossom Street, Boston MA 02114
    • Wound Healing Laboratory, Shriners Burns Institute, 51 Blossom Street, Boston MA 02114
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  • Dr. Roger R. Markwald

    1. Department of Anatomy, Texas Tech University Health Sciences Center, Lubbock, TX 79430
    Current affiliation:
    1. Department of Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226
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Abstract

A collagen-lattice culture model of developing heart valves was utilized to test two glycosaminoglycans, normally found in the cardiac jelly matrix of developing heart valve primordia, for their effects on the capability of mesenchymal derivatives of cardiac cushion endothelial cells to enter the substrate from the surface. Treatment with hyaluronate increased the rate of cell seeding to 2.04 times that of untreated control cultures and 1.82 times that of chondroitin sulfate-treated cultures. Scanning electron microscopic studies suggested that the increased rate was due to an enhanced disruption of intercellular junctions, influenced by hyaluronate, permitting disengagement of cells from the surface population and migration as mesenchymal cells into the collagen matrix. The results of this study correlate well with the presence of high hyaluronate concentrations in the cardiac jelly matrix beneath the cushion endothelium at periods of active seeding of cushion tissue cells.

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