General Histology and Cytology
Calcium-binding protein (28,000 Mr calbindin-D28k) in kidneys of the bullfrog Rana catesbeiana during metamorphosis
Article first published online: 1 FEB 2005
Copyright © 1986 Wiley-Liss, Inc.
The Anatomical Record
Volume 216, Issue 2, pages 127–132, October 1986
How to Cite
Rhoten, W. B., Gona, O. and Christakos, S. (1986), Calcium-binding protein (28,000 Mr calbindin-D28k) in kidneys of the bullfrog Rana catesbeiana during metamorphosis. Anat. Rec., 216: 127–132. doi: 10.1002/ar.1092160203
- Issue published online: 1 FEB 2005
- Article first published online: 1 FEB 2005
- Manuscript Accepted: 16 APR 1986
- Manuscript Received: 5 NOV 1985
A protein of approximately 28,000 relative molecular mass (Mr) cross-reacting with antiserum against the 28,000-Mr rat renal calcium-binding protein (calbindin-D28k) has been localized in the kidney of a salientian amphibian, Rana catesbeiana. Cells reactive for calbindin-D28k were found in the distal tubule at all stages of metamorphosis by the unlabeled antibody peroxidase-antiperoxidase technique. Adult kidneys appeared to have more calbindin-D28k-positive cells. The renal corpuscle, neck, and proximal tubule were negative. An immunoreactive 28,000-Mr band that comigrated with the band of calbindin-D28k was visualized by the immunoblot technique. The finding of the 28,000-Mr calbindin-D in the anamniotic kidney demonstrates that this calcium-binding protein (CaBP) is phylogenetically older than our previous studies of higher vertebrates had revealed (Rhoten et al., 1985). Although the function of calbindin-D28k in the distal nephron is unknown, this CaBP can now be presumed to have functional significance in the mesonephric as well as the metanephric kidney.