Deformation-corrected computer-aided three-dimensional reconstruction of immunohistochemically stained organs: Application to the rat heart during early organogenesis
Article first published online: 26 JAN 2005
Copyright © 1989 Wiley-Liss, Inc.
The Anatomical Record
Volume 224, Issue 3, pages 443–457, July 1989
How to Cite
Laan, A. C., Lamers, W. H., Huijsmans, D. P., Kortschot, A. T., Smith, J., Strackee, J. and Los, J. A. (1989), Deformation-corrected computer-aided three-dimensional reconstruction of immunohistochemically stained organs: Application to the rat heart during early organogenesis. Anat. Rec., 224: 443–457. doi: 10.1002/ar.1092240313
- Issue published online: 26 JAN 2005
- Article first published online: 26 JAN 2005
- Manuscript Accepted: 3 OCT 1988
- Manuscript Received: 23 JUN 1988
The application of a computer-assisted, three-dimensional reconstruction procedure for serial sections to embryonic rat hearts during the period of cardiac looping and compartmentation is described. The procedure relies on immunohistochemical staining for the introduction of selective contrast and on episcopic and diascopic images of each of the sections for alignment and correction of compression due to sectioning. Episcopic (reference) images are taken from the embedding block just before the cutting of a slice and are still aligned and undeformed. Diascopic images are taken from the sections after immunohistochemical processing and, hence, contain selective contrast but are deformed and no longer aligned. The three-dimensional images are visualized as shaded voxel models.
This approach allowed the unequivocal delineation of the developing myocardium and the inspection of its changing architecture both from the outside and from within. Furthermore, it allowed a quantification of myocardial volume. Because standardized and hence comparable views of three different stages were generated, changes in the shape of the cardiac loop, the atria, and the ventricles as well as changes in the position of the atrioventricular canal and interventricular foramen could be accurately described. Characteristic changes in the position of both the right ventricle and the atrioventricular canal that are essential for the formation of a correctly functioning four-chambered heart could be observed. These changes in shape occur while the myocardial size increases dramatically.