Spatial distribution of “tissue-specific” antigens in the developing human heart and skeletal muscle. I. An immunohistochemical analysis of creatine kinase isoenzyme expression patterns

Authors

  • A. Wessels,

    1. Department of Anatomy and Embryology, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
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  • J. L. M. Vermeulen,

    1. Department of Anatomy and Embryology, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
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  • S. Z. Virágh,

    1. Department of Pathology, Postgraduate Medical School, Budapest 112, Hungary 1389
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  • F. Kálmán,

    1. Department of Pathology, Postgraduate Medical School, Budapest 112, Hungary 1389
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  • G. E. Morris,

    1. Department of Research Division, North East Wales Institute, Deeside, Clwyd CH5 4BR, United Kingdom
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  • Nguyen Thi Man,

    1. Department of Research Division, North East Wales Institute, Deeside, Clwyd CH5 4BR, United Kingdom
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  • W. H. Lamers,

    1. Department of Anatomy and Embryology, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
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  • A. F. M. Moorman

    Corresponding author
    1. Department of Anatomy and Embryology, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    • Department of Anatomy and Embroyology University of Amsterdam, Acadmic Medical Centre, Meibergdreef 15, 1105 AZ Amsterdam, The Nehterlands
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Abstract

Using monoclonal antibodies against the M and B subunit isoforms of creatine kinase (CK) we have investigated their distribution in developing human skeletal and cardiac muscle immunohistochemically. It is demonstrated that in skeletal muscle, a switch from CK-B to CK-M takes place around the week 8 of development, whereas in the developing heart, CK-M is the predominant isoform from the earliest stage examined onward (i.e., 4½ weeks of development). In all hearts examined, local differences in concentration of the CK isoforms are observed. The CK-M expression in the developing outflow tract (OFT) and conduction system is described in detail. Between the weeks 5 and 7 of development, the distal portion of the OFT is characterized by low CK-M expression, whereas around the week 8–10 of development the myocardium around the developing semilunar valves in the OFT expresses a very high level of CK-M. At all stages examined, a relatively low CK-M level is observed in those regions in which the “slow” components of the conduction system do develop (e.g., the sinoatrial junction and atrioventricular junction), whereas a relatively high concentration of CK-M is observed in those areas that are destined to become the “fast” components, i.e., the subendocardial myocardium of the ventricles. The high expression of CK-M in the developing “fast components” of the conduction system contrasts with the relatively low expression of CK-M in the force-producing myocardium of the interventricular septum and free ventricular wall.

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