Fiducial points for three-dimensional computer-assisted reconstruction of serial light microscopic sections of umbilical cord

Authors

  • Ivano Ongaro,

    1. Department of Oral Biology, Faculty of Dentistry, University of Alberta, Edmonton, Alberta, Canada
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  • Dr. Geoffrey H. Sperber,

    Corresponding author
    1. Department of Oral Biology, Faculty of Dentistry, University of Alberta, Edmonton, Alberta, Canada
    • Department of Oral Biology, Faculty of Dentistry, University of Alberta, Edmonton, Alberta, T6G 2N8 Canada
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  • Geoffrey A. Machin,

    1. Department of Oral Biology, Faculty of Dentistry, University of Alberta, Edmonton, Alberta, Canada
    2. Department of Pathology, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada
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  • Colleen A. Murdoch

    1. Department of Oral Biology, Faculty of Dentistry, University of Alberta, Edmonton, Alberta, Canada
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Abstract

Fascicles of human sural (peripheral sensory) nerve were used as external fiducial points (FP) for accurate registration of serial light microscopic sections in three-dimensional reconstruction of human umbilical cord. This paper describes a method for embedding the FPs within the paraffin wax block simultaneously with the specimen to be sectioned. Using a new design of embedding box, the FPs are embedded close to the specimen and are transferred to the slides as part of the tissue sections. Three to four FPs were used to align and scale the serial tissue sections for digitization and computerized reconstruction, using a commercially available software program on IBM-compatible 80386 hardware. A three-dimensional solid surface graphic model of a segment of human umbilical cord was generated.

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