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Immunobiology
Serotonin-like immunoreactivity in Merkel cells and their afferent neurons in touch domes from the hairy skin of rats
Article first published online: 26 JAN 2005
DOI: 10.1002/ar.1092320112
Copyright © 1992 Wiley-Liss, Inc.
1932-8494/asset/cover.gif?v=1&s=f95bde22395f72795e3dd8e55db6c7f940a1a957 "The Anatomical Record")
Additional Information
How to Cite
English, K. B., Wang, Z.-Z., Stayner, N., Stensaas, L. J., Martin, H. and Tuckett, R. P. (1992), Serotonin-like immunoreactivity in Merkel cells and their afferent neurons in touch domes from the hairy skin of rats. Anat. Rec., 232: 112–120. doi: 10.1002/ar.1092320112
Publication History
- Issue published online: 26 JAN 2005
- Article first published online: 26 JAN 2005
- Manuscript Accepted: 10 MAY 1991
- Manuscript Received: 22 MAR 1991
- Abstract
- References
- Cited By
Abstract
Immunoreactivity to serotonin was observed in Merkel cells as well as the afferent type I nerves terminating upon them in touch domes excised from the belly skin of rats. Type I nerves were strongly immunoreactive and could be traced through the dermis of the domal papilla. Merkel cell immunoreactivity was sometimes seen in the entire cell, but was often localized in the Merkel cell cytoplasm adjacent to nerve terminals and may have been in the terminals themselves. Domes were fixed by immersion in 4% paraformaldehyde-lysine-sodiumm-periodate (PLP) fixative at 4°C for 2.5–3 hours and cryoprotected in 30% sucrose overnight. Sections were processed with the avidin-biotin complex peroxidase (ABC), peroxidase-antiperoxidase (PAP), and indirect immunofluorescence techniques with rabbit antiserum generated against serotonin.