Effects of prolactin on α and β chloride cells in the gill epithelium of the saltwater adapted tilapia “Oreochromis niloticus”
Article first published online: 26 JAN 2005
Copyright © 1993 Wiley-Liss, Inc.
The Anatomical Record
Volume 235, Issue 2, pages 275–284, February 1993
How to Cite
Pisam, M., Auperin, B., Prunet, P., Rentier-Delrue, F., Martial, J. and Rambourg, A. (1993), Effects of prolactin on α and β chloride cells in the gill epithelium of the saltwater adapted tilapia “Oreochromis niloticus”. Anat. Rec., 235: 275–284. doi: 10.1002/ar.1092350211
- Issue published online: 26 JAN 2005
- Article first published online: 26 JAN 2005
- Manuscript Accepted: 25 JUN 1992
- Manuscript Received: 3 APR 1992
- Chloride cells
Tilapia (Oreochromis niloticus), 21 g average body weight, were divided into two groups. A group was maintained in fresh water, whereas another group was adapted for 2 weeks to 20% salt water. Among the latter, fishes were injected every 2 days for a week with tilapia prolactin (ti-PRL I). Gills were prepared for electron microscopy in order to determine the types and surface areas of chloride cells in each experimental condition. Two types of chloride cells, the α and β cells were easily distinguished on the basis of their location and ultrastructural features in the gills of freshwater fishes, while only one type of cell, the saltwater α cells presumably derived from the transformation of the freshwater α cells, were encountered in saltwater adapted animals. After PRL injection ofsaltwater adapted fishes, small chloride cells, which displayed ultrastructural features similar to those of β cells in freshwater tilapia, reappeared in interlamellar regions of the gills. In the same experimental conditions, the voluminous saltwater α cells showed a tendency to resume ultrastructural features more characteristic of the freshwater α cells from which they were derived. These observations tend to indicate that prolactin behaves as a “freshwater adapting hormone” and that β cells are specifically involved in fish adaptation to freshwater living conditions. © 1993 Wiley-Liss, Inc.