Dynamics of epithelial cells in the corpus of the mouse stomach. III. Inward migration of neck cells followed by progressive transformation into zymogenic cells

Authors

  • Sherif M. Karam,

    1. Department of Anatomy, McGill University, Montreal, Quebec, Canada
    Current affiliation:
    1. Department of Molecular and Cellular Biology, 245 Life Sciences Addition, University of California, Berkeley, CA 94720
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  • Dr. Charles Philippe Leblond

    Corresponding author
    1. Department of Anatomy, McGill University, Montreal, Quebec, Canada
    • Department of Anatomy, McGill University, 3640 University St., Montreal, Quebec, Canada H3A 2B2
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Abstract

The neck cells (or mucous neck cells) present in the neck region and the zymogenic cells (or chief cells) present in the base region of the units in the mouse corpus were examined in the electron microscope (EM) and in radioautographs prepared after administration of 3H-thymidine by single or multiple injections or by continuous infusion for 1–52 days. For these studies, the neck region of the units has been subdivided into three equal segments, respectively named high neck, mid neck, and low neck, while the base region has been similarly subdivided into high base, mid base, and low base.

The neck region includes an average of 12.6 neck cells, characterized in the EM by dark, mucous secretory granules that frequently exhibit a light, pepsinogenic core. Continuous 3H-thymidine infusion reveals that neck cells come from pre-neck cells, which are believed to arise in the isthmus region from the undifferentiated granule-free cells through a pre-neck cell precursor stage. The pre-neck cells, characterized by the presence of a few cored secretory granules, migrate inward (i.e., in the direction of the blind end of the units) and enter the neck region to become neck cells. It is estimated that 59% of the neck cells arise from differentiation of pre-neck cells, whereas the other 41% are derived from their own mitoses. Neck cells migrate inward in 1–2 weeks from the high through the mid and low neck segments, while they keep on producing more and larger secretory granules and thus further differentiate as mucus-producing cells.

When neck cells reach the high base segment, they become pre-zymogenic cells that produce secretory granules in which appear light, irregular, pepsinogenic patches which encroach on the dark mucous content. With time, the pre-zymogenic cells, of which there are 5.0 per unit on the average, keep on producing new granules with larger and larger light patches, so that in the end the cells produce granules which are entirely filled by light, pepsinogenic material. At this stage, the cells are zymogenic cells.

Zymogenic cells, which average 67.5 per unit, further migrate inward, while gradually enlarging and producing pepsinogenic granules of increasing size. In the low base segment, some zymogenic cells show signs of degeneration leading to death by either necrosis or apoptosis. While remnants of the necrotic cells appear to be released to the unit lumen, the apoptotic cells are phagocytosed by a neighboring zymogenic cell or by a connective tissue macrophage breaking through the basement membrane of the oxyntic unit.

Briefly, the zymogenic cell lineage, a cell sequence initiated from the stationary undifferentiated granule-free cells, includes pre-neck cell pre-cursors, pre-neck cells, neck cells, pre-zymogenic cells, and finally zymogenic cells, which all migrate in the direction of the unit's blind end, near which zymogenic cells are lost. © 1993 Wiley-Liss, Inc.

Ancillary