Drs. Shan and Shen have contributed equally to this work.
pCREB is Involved in Neural Induction of Mouse Embryonic Stem Cells by RA
Version of Record online: 27 MAR 2008
Copyright © 2008 Wiley-Liss, Inc.
The Anatomical Record
Volume 291, Issue 5, pages 519–526, May 2008
How to Cite
Shan, Z.-Y., Shen, J.-L., Li, Q.-M., Wang, Y., Huang, X.-Y., Guo, T.-Y., Liu, H.-W., Lei, L. and Jin, L.-H. (2008), pCREB is Involved in Neural Induction of Mouse Embryonic Stem Cells by RA. Anat Rec, 291: 519–526. doi: 10.1002/ar.20686
- Issue online: 8 APR 2008
- Version of Record online: 27 MAR 2008
- Manuscript Accepted: 22 JAN 2008
- Manuscript Received: 30 JUL 2007
- National Natural Science Foundation of China. Grant Number: 30671025
- The National Key Technologies R&D Program of China during the 10th Five-Year Plan Period. Grant Number: 2004DA754C
- Foundation of Heilongjiang Province Educational Committee for Returnees. Grant Number: 1151h2031
- Department of Health of Heilongjiang Province
- stem cells;
- neural progenitor cells;
Mouse embryonic stem (ES) cells can be induced by various chemicals to differentiate into a variety of cell types in vitro. In our study, retinoic acid (RA), one of the most important inducers, used at a concentration of 5 μM, was found to induce the differentiation of ES cells into neural progenitor cells (NPCs). During embryoid body (EB) differentiation, the level of active cyclic AMP response element-binding protein (CREB) was relatively high when 5 μM RA treatment was performed. Inhibition of CREB activity committed EBs to becoming other germ layers, whereas increased expression of CREB enhanced NPC differentiation. Moreover, RA increased the expression of active CREB by enhancing the activity of JNK. Our research suggests that CREB plays a role in RA-induced NPC differentiation by increasing the expression of active JNK. Anat Rec, 291:519–526, 2008. © 2008 Wiley-Liss, Inc.