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Spatiotemporal Expression of SSeCKS in Injured Rat Sciatic Nerve

  1. Li Chen1,2,†,
  2. Jing Qin2,†,
  3. Chun Cheng3,
  4. Shuqiong Niu1,
  5. Yonghua Liu3,
  6. Shuxian Shi3,
  7. Hai'ou Liu3,
  8. Aiguo Shen1,‡,*

Article first published online: 2 APR 2008

DOI: 10.1002/ar.20692

Issue

The Anatomical Record

The Anatomical Record

Volume 291, Issue 5, pages 527–537, May 2008

Additional Information

How to Cite

Chen, L., Qin, J., Cheng, C., Niu, S., Liu, Y., Shi, S., Liu, H. and Shen, A. (2008), Spatiotemporal Expression of SSeCKS in Injured Rat Sciatic Nerve. Anat Rec, 291: 527–537. doi: 10.1002/ar.20692

Author Information

  1. 1

    The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, People's Republic of China

  2. 2

    Department of Pathology, Medical School of Nantong University (Former Nantong Medical College), Nantong, People's Republic of China

  3. 3

    Department of Microbiology and Immunology, Medical School of Nantong University (Former Nantong Medical College), Nantong, People's Republic of China

  1. †

    Drs. Chen and Qin contributed equally to this work.

  2. ‡

    Fax: 86-513-85051999

*The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, People's Republic of China, 226001

  1. †

    Drs. Chen and Qin contributed equally to this work.

  2. ‡

    Fax: 86-513-85051999

Publication History

  1. Issue published online: 8 APR 2008
  2. Article first published online: 2 APR 2008
  3. Manuscript Accepted: 17 JAN 2008
  4. Manuscript Received: 1 SEP 2007

Funded by

  • The National Natural Scientific Foundation of China. Grant Numbers: 30300099, 30770488
  • Natural Scientific Foundation of Jiangsu Province. Grant Numbers: BK2003035, BK2006547

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Keywords:

  • SSeCKS;
  • sciatic nerve;
  • crush;
  • transect;
  • rat

Abstract

SSeCKS (src suppressed C kinase substrate) functions in the control of cell signaling and cytoskeletal arrangement. It is expressed in brain and spinal cord, but little is known about its expression in peripheral nerves. In this study, in rats, real-time polymerase chain reaction and Western blot analysis showed that expression of SSeCKS in crushed sciatic nerve reached its highest level 6 hr after crushing, whereas in a transection model, SSeCKS peaked at 2 days in the proximal stump and 12 hr in the distal stump. Immunohistochemical analysis demonstrated up-regulation of SSeCKS protein surrounding the crush site and in the two stumps of the transected nerve. In addition, SSeCKS colocalized with growth-associated protein 43 and with S100, which also changed with time after injury. These findings support the idea that SSeCKS participates in the adaptive response to peripheral nerve injury and may be associated with regeneration. Anat Rec, 291:527–537, 2008. © 2008 Wiley-Liss, Inc.

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