Assessment of Islet Graft Survival Using a 3.0-Tesla Magnetic Resonance Scanner

Authors

  • Yang Jiao,

    1. Shanghai Organ Transplantation Clinical Medical Center, the First People's Hospital affiliated Shanghai Jiao Tong University, Shanghai, China
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  • Zhi-Hai Peng,

    Corresponding author
    1. Shanghai Organ Transplantation Clinical Medical Center, the First People's Hospital affiliated Shanghai Jiao Tong University, Shanghai, China
    • Shanghai Organ Transplantation Clinical Center, the First People's Hospital affiliated Shanghai Jiao Tong University, No. 100 Hai'ning Road, Shanghai 200080, China
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    • Fax: 021-63240825.

  • Tong-Hai Xing,

    1. Shanghai Organ Transplantation Clinical Medical Center, the First People's Hospital affiliated Shanghai Jiao Tong University, Shanghai, China
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  • Jie Qin,

    1. Department of Anatomy, Histology and Embryology, Shanghai Medical College, Fudan University, Shanghai, China
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  • Cui-Ping Zhong

    1. Department of Anatomy, Histology and Embryology, Shanghai Medical College, Fudan University, Shanghai, China
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Abstract

Some studies have recently described a magnetic resonance (MR) method for detection of iron-labeled islets transplanted into the liver. The aim of this work was to assess the survival of islet graft using a clinical 3.0-T scanner. Islets from Lewis rats were cultured in the presence of iron oxide nanoparticles. One thousand iron-labeled islets were transplanted into the portal vein of diabetic rats. Blood glucose levels were measured daily through day 14 post-transplantation. MR imaging of the same section of the liver was performed on 1, 3, 7, 10, and 14 days post-transplantation. The labeled islets were visualized by MR as distinct hypointensive spots distributed in the liver. There was a linear correlation between the relative value of delta Rmath image relaxometry multiplied by the cubic diameter (relative value of the iron volume, Ir) and blood glucose level on 14 days post-transplantation in allograft and isograft (P < 0.05). The relative value of delta Rmath image relaxometry, diameter, and number of hypointensive spots could be calculated to assess the survival of the iron-labeled islet grafts. Assessment of iron-labeled islet grafts using a clinical 3.0-T magnetic resonance scanner represents a useful method that has potential for clinical use. Anat Rec, 2008. © 2008 Wiley-Liss, Inc.

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