Expression of KiSS-1 Gene and its Role in Invasion and Metastasis of Human Hepatocellular Carcinoma
Article first published online: 30 JUL 2009
Copyright © 2009 Wiley-Liss, Inc.
The Anatomical Record
Volume 292, Issue 8, pages 1128–1134, August 2009
How to Cite
Shengbing, Z., Jing Feng, L., Bin, W., Lingyun, G. and Aimin, H. (2009), Expression of KiSS-1 Gene and its Role in Invasion and Metastasis of Human Hepatocellular Carcinoma. Anat Rec, 292: 1128–1134. doi: 10.1002/ar.20950
- Issue published online: 30 JUL 2009
- Article first published online: 30 JUL 2009
- Manuscript Accepted: 24 MAY 2009
- Manuscript Received: 12 MAR 2009
- Key Project of Chinese Ministry of Education. Grant Number: 205077
- Foundation of Fujian Educational Committee. Grant Number: JS06011
- liver neoplasm;
- tissue chip
KiSS-1 has been identified as a putative metastasis-suppressor gene in human melanomas and breast cancer cell lines. Although loss of KiSS-1 expression has been associated with progression and poor prognosis of various cancers, the exact role of KiSS-1 expression in HCC is not well-defined. Our study investigated KiSS-1 expression levels in HCC and its role in invasion and metastasis of human HCC. The expression levels of KiSS-1 and MMP-9 protein were determined by tissue microarray (TMA) serial sections, immunohistochemistry and semi-quantitative image analysis. All clinical and histological data obtained were subjected to statistical analysis. The expression of KiSS-1 protein in HCC and intrahepatic metastasis lesions was significantly lower (P < 0.01) when compared with non-tumor liver tissue and normal liver tissue. Multivariate analysis revealed a significant inverse correlation between KiSS-1 expression and ○1 TNM stage, (F = 7.113, P < 0.01) and ○2intrahepatic metastasis (t = 2.898, P < 0.01). Loss of KiSS-1 in intrahepatic metastasis versus primary carcinomas was statistically significant (P<0.01). We also found a negative correlation between KiSS-1 and MMP-9 expression in HCC (r = -0.506, P < 0.01). We conclude that loss of KiSS-1 during HCC metastasis, along with a concomitant upregulation of MMP-9 suggests a possible mechanism for cell motility and invasion during HCC metastasis, with KiSS-1 emerging as a possible therapeutic target during HCC metastasis. Anat Rec, 292:1128–1134, 2009. © 2009 Wiley-Liss, Inc.