Blood Flow Patterns Spatially Associated with Platelet Aggregates in Murine Colitis
Article first published online: 30 JUL 2009
Copyright © 2009 Wiley-Liss, Inc.
The Anatomical Record
Volume 292, Issue 8, pages 1143–1153, August 2009
How to Cite
Miele, L. F., Turhan, A., Lee, G. S., Lin, M., Ravnic, D., Tsuda, A., Konerding, M. A. and Mentzer, S. J. (2009), Blood Flow Patterns Spatially Associated with Platelet Aggregates in Murine Colitis. Anat Rec, 292: 1143–1153. doi: 10.1002/ar.20954
- Issue published online: 30 JUL 2009
- Article first published online: 30 JUL 2009
- Manuscript Accepted: 17 MAR 2009
- Manuscript Received: 31 DEC 2008
- NIH. Grant Numbers: HL47078, HL75426, HL054885, HL074022, HL070542
- blood flow;
- intravital microscopy
In the normal murine mucosal plexus, blood flow is generally smooth and continuous. In inflammatory conditions, such as chemically-induced murine colitis, the mucosal plexus demonstrates markedly abnormal flow patterns. The inflamed mucosal plexus is associated with widely variable blood flow velocity as well as discontinuous and even bidirectional flow. To investigate the mechanisms responsible for these blood flow patterns, we used intravital microscopic examination of blood flow within the murine mucosal plexus during dextran sodium sulphate-and trinitrobenzenesulfonic acid-induced colitis. The blood flow patterns within the mucosal plexus demonstrated flow exclusion in 18% of the vessel segments (P < 0.01). Associated with these segmental exclusions was significant variation in neighboring flow velocities. Intravascular injection of fluorescent platelets demonstrated platelet incorporation into both fixed and rolling platelet aggregates. Rolling platelet aggregates (mean velocity 113 μm/sec; range, 14–186 μm/sec) were associated with reversible occlusions and flow variations within the mucosal plexus. Gene expression profiles of microdissected mucosal plexus demonstrated enhanced expression of genes for CCL3, CXCL1, CCL2, CXCL5, CCL7, CCL8, and Il-1b (P < 0.01), and decreased expression of CCL6 (P < 0.01). These results suggest that platelet aggregation, activated by the inflammatory mileau, contributes to the complex flow dynamics observed in acute murine colitis. Anat Rec, 292:1143–1153, 2009. © 2009 Wiley-Liss, Inc.