Blood Flow Patterns Spatially Associated with Platelet Aggregates in Murine Colitis
Article first published online: 30 JUL 2009
Copyright © 2009 Wiley-Liss, Inc.
The Anatomical Record
Volume 292, Issue 8, pages 1143–1153, August 2009
How to Cite
Miele, L. F., Turhan, A., Lee, G. S., Lin, M., Ravnic, D., Tsuda, A., Konerding, M. A. and Mentzer, S. J. (2009), Blood Flow Patterns Spatially Associated with Platelet Aggregates in Murine Colitis. Anat Rec, 292: 1143–1153. doi: 10.1002/ar.20954
- Issue published online: 30 JUL 2009
- Article first published online: 30 JUL 2009
- Manuscript Accepted: 17 MAR 2009
- Manuscript Received: 31 DEC 2008
- NIH. Grant Numbers: HL47078, HL75426, HL054885, HL074022, HL070542
- blood flow;
- intravital microscopy
In the normal murine mucosal plexus, blood flow is generally smooth and continuous. In inflammatory conditions, such as chemically-induced murine colitis, the mucosal plexus demonstrates markedly abnormal flow patterns. The inflamed mucosal plexus is associated with widely variable blood flow velocity as well as discontinuous and even bidirectional flow. To investigate the mechanisms responsible for these blood flow patterns, we used intravital microscopic examination of blood flow within the murine mucosal plexus during dextran sodium sulphate-and trinitrobenzenesulfonic acid-induced colitis. The blood flow patterns within the mucosal plexus demonstrated flow exclusion in 18% of the vessel segments (P < 0.01). Associated with these segmental exclusions was significant variation in neighboring flow velocities. Intravascular injection of fluorescent platelets demonstrated platelet incorporation into both fixed and rolling platelet aggregates. Rolling platelet aggregates (mean velocity 113 μm/sec; range, 14–186 μm/sec) were associated with reversible occlusions and flow variations within the mucosal plexus. Gene expression profiles of microdissected mucosal plexus demonstrated enhanced expression of genes for CCL3, CXCL1, CCL2, CXCL5, CCL7, CCL8, and Il-1b (P < 0.01), and decreased expression of CCL6 (P < 0.01). These results suggest that platelet aggregation, activated by the inflammatory mileau, contributes to the complex flow dynamics observed in acute murine colitis. Anat Rec, 292:1143–1153, 2009. © 2009 Wiley-Liss, Inc.
In the normal murine mucosal plexus, blood flow is generally smooth and continuous. In inflammatory conditions, such as chemically-induced murine colitis, the mucosal plexus demonstrates markedly abnormal flow patterns. The inflamed mucosal plexus is associated with not only widely variable changes in blood flow velocity (Ravnic et al.,2007b), but discontinuous and even bidirectional flow (Turhan et al.,2007; Tsuda et al., in press). These flow patterns in colitis are relevant to understanding not only metabolic supply and demand, but also flow-induced alterations in gene expression (Zhao et al.,2002; Chien,2007).
Attempts to explain the vascular events in inflammatory bowel disease have focused on small vessel thrombosis. In regional enteritis, mucosal vascular changes ranging from fibrin deposition to complete thrombotic occlusions occurs early in the disease evolution and precedes mucosal ulceration (Wakefield et al.,1989). Platelets have been implicated in the capillary occlusion by immunostaining for the platelet glycoprotein IIIa (Hudson et al.,1993). Similarly, platelet-associated thrombotic changes have been implicated in the pathogenesis of colitis. Biopsies of patients with ulcerative colitis have identified intravascular platelet aggregates (Donnellan,1966) and intracapillary thrombus (Dhillon et al.,1992). Murine models of chemically-induced colitis have demonstrated increased platelet adhesions in the inflammatory mucosal vessels (Mori et al.,2005a; Anthoni et al.,2006). Platelet adhesions to vessel walls have been correlated with both disease activity and vascular permeability (Mori et al.,2005b) and appear to be mediated, at least in part, by the platelet adhesion molecule P-selectin (Anthoni et al.,2006; Rivera-Nieves et al.,2006; Rijcken et al.,2007; Vowinkel et al.,2007b). Finally, the relevance of platelet activation is supported by studies of the CD40-CD40L signaling pathway in both patients (Danese et al.,2003) and murine models of colits (Vowinkel et al.,2007a) Despite the growing evidence of platelet activation in the mucosal plexus, the effect of platelet aggregation on intramucosal blood flow patterns is unknown.
In this report, we investigated the blood flow patterns within the inflammatory colon microcirculation. Blood flow dynamics in the mucosal plexus were spatially associated with platelet aggregates and functionally implicated in both fixed and reversible flow exclusion. The mechanism of platelet activation was explored using RT-PCR arrays that demonstrated the expression of multiple platelet agonists within the inflamed mucosal plexus.
C57B/6 and BALB/c mice (Jackson Laboratory, Bar Harbor, ME), 25–33 g, were used in all experiments. The care of the animals was consistent with guidelines of the American Association for Accreditation of Laboratory Animal Care (Bethesda, MD) and approved by the Institutional Animal Care and Use Committee.
Dextran Sulfate Administration
The dextran sodium sulfate (DSS) (TdB Consultancy AB, Uppsala, Sweden) model of colitis was similar to that described previously (Okayasu et al.,1990). Briefly, DSS was freshly prepared and added daily to the BALB/c mice drinking water at a final concentration of 5%. The mice were assessed daily for clinical signs and total body weight. The DSS treatment was continued for 7 days. The mice were studied on days 7 to 65. Acute colitis was defined as 7 to 10 days after DSS exposure.
Trinitrobenzenesulfonic Acid Administration
The 2,4,6-Trinitrobenzenesulfonic acid (TNBS) (Sigma, St. Louis, MO) model of colitis was similar to that described previously (Ravnic et al.,2007a). After the mouse abdomen was sheared and cleansed with water, 36 μL of a 2.5% 2,4,6-Trinitrochlorobenzene (TNCB) (ChemArt, Egling, Germany) in a 4:1 acetone:olive oil solution was sprayed onto a 1.5 cm diameter circular PhastTansfer Filter Paper (Pharmacia, Upsala, Sweden). The TNCB soaked filter paper was applied to the sheared abdomen and secured with Tegaderm (3M, St. Paul, MN) and Durapore Surgical Tape (3M, St. Paul, MN). The TNCB patch was removed 24 hrs after application. On postsensitization day six, 125 μL of 1.75% TNBS in a 50% ethanol solution was instilled into the rectum. Control mice had only the 50% ethanol solution instilled intrarectally. Acute colitis was defined as 5 to 7 days after TNBS exposure.
Scanning Electron Microscopy
After systemic heparinization, PBS perfusion and intravascular fixation with 2.5% buffered glutaraldehyde, the systemic circulation was perfused with 10–20 mL of Mercox (SPI, West Chester, PA) diluted with 20% methyl methacrylate monomers (Aldrich Chemical, Milwaukee, WI) as described previously (Konerding et al.,2001; Ravnic et al.,2005). After complete polymerization, the tissues were harvested and macerated in 5% potassium hydroxide followed by drying and mounting for scanning electron microscopy. The microvascular corrosion casts were imaged after coating with gold in an argon atmosphere with a Philips ESEM XL30 scanning electron microscope (Eindhoven, Netherlands) (Konerding et al.,1998).
The colon was exteriorized through a midline laparotomy incision and imaged using a Nikon Eclipse TE2000 inverted epifluorescence microscope using Nikon water dipping Fluor 10×, 20×, and 40× objectives. The intravital microscopy was performed by using a custom-machined immersion stage. The tissue contact area consisted of vacuum galleries that provided tissue apposition to the lens surface without tissue compression. An X-Cite (Exfo; Vanier, Canada) 120 watt metal halide light source and a liquid light guide was used to illuminate the tissue samples. Excitation and emission filters (Chroma, Rockingham, VT) in separate LEP motorized filter wheels were controlled by a MAC 5000 controller (Ludl, Hawthorne, NY) and MetaMorph Imaging System 7.5 software (MDS Analytical Technologies, Brandywine, PA). The intravital videomicroscopy 14-bit fluorescent images were digitally recorded with an electron multiplier CCD (EMCCD) camera (C9100-02, Hamamatsu, Japan). Images with 1,000× 1,000 pixel resolution were routinely obtained at frame rates exceeding 60 fps with 2 × 2 binning or subarray acquisition. The images were recorded in image stacks comprising 30 sec to 10 min video sequences on a Dell Precision workstation (3.06 Ghz Xeon processors, 15,000 rpm ultra-SCSI hard drive, 4 GB RAM and an Nvidia Quadro 3,450 graphics card with 512 mb memory). The image stacks were processed with standard MetaMorph filters.
Morphometry and Image Analysis
Images were processesed with the MetaMorph 7.5 software (MDS Analytical Technologies). The 14-bit grayscale images were thresholded and standard distance calibration was performed. The MetaMorph's region measurements and caliper applications were used to measure platelet aggregates. Routine distance calibration and thresholding were applied to the “stacked” image sequences (Ravnic et al.,2006b). The data was logged into Microsoft Excel 2003 (Redmond, WA) by dynamic data exchange.
Platelet Isolation and Fluorochrome Labeling
Platelet procedures were designed to avoid temperature variation or platelet agitation. After general anesthesia, 900 μL of donor blood was obtained in a syringe filled with 0.1 mL of citate-dextrose solution (Sigma, St Louis). The blood was sequentially centrifuged at 200, 500, and 1000g for 10 min followed by removal of the platelet rich plasma layer. The pooled platelet fractions were resuspended in 750 μL of phosphate buffered saline. A 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen, Eugene, OR) fluorochrome solution was added to platelets at a final concentration of 125 μM and incubated for 10 min at 25°C. The platelets were washed in a 10% citrate-dextrose solution and resuspended in 0.5 mL of phosphate buffered saline. Typical platelet concentrations were 4.0–6.5 × 108/mL. The CFSE-labeled platelets (310 μL) were injected into the mouse tail vein.
Intravenous Fluorescence Labeling
In vivo fluorescence labeling of circulating blood elements was performed with CFSE. CFSE (Invitrogen, Eugene, OR) labeling solution was prepared in DMSO as described (Becker et al.,2004; Ravnic et al.,2006a). The freshly prepared CFSE (400 μL) was injected into the mouse tail vein over 2–3 minutes. The CFSE tracer (ex 480 nm, em 520 nm) was imaged with 25 nm band pass filters (Omega).
Fluorescent Intravascular Tracers
The previously described 500 nm polystyrene spheres were used to visualize flow fields by intravital microscopy (Ravnic et al.,2006a,b). The particles were labeled with derivatives of the BODIPDY fluorochrome using organic solvents (Invitrogen; Eugene OR). The nanoparticles used in this study were typically green (ex 488; em 510); however, orange (ex 545 nm; em 570 nm) and infra-red (ex 655 nm; em 710 nm) tracers were also used.
Isotropic Line Probes
Systematic and random sampling of tracer flow paths in the mucosal plexus was performed using isotropic line probes. A digital grid consisting of four parallel flow paths was overlaid on each intravital microscopy recording. The lines were shifted a random fraction of the spacing between lines, then rotated to an angle given by a random number between 0 and 180. The flow path most closely corresponding to the line probe was recorded. Optical resolution suitability of each segment of the flow path was assessed before data collection; segments without sufficient optical resolution were excluded from the data collection.
Tracer Flux Variability
A space-time plot was drawn in each vessel segment through each random flow path using the MetaMorph 7.5 (MDS Analytical Technologies) kymograph application. Flux was calculated for each vessel segment as the number of tracers per unit time; thus, flux was independent of flow direction. Flux graphically represented on a stepcurve with each step reflecting a successive vessel segment. Variability of the flux was quantified using analysis of variance.
Multiframe Particle Tracking
Tracking of the intravascular tracers was performed on digitally recorded and distance calibrated multi-image “stacks” (Ravnic et al.,2006a). The image stacks produced a sequential time history of velocity and direction as the acquired images were time stamped based on the 100 mhz system bus clock of the Xeon processor (Intel, Santa Clara, CA). The movement of individual particles was tracked using the MetaMorph 7.5 (MDS Analytical Technologies) object tracking application. For displacement reference, the algorithm used the location of the particle at its first position in the stack. Each particle was imaged as a high contrast fluorescent disk and its position was determined with sub pixel accuracy. The image of the particle was tracked using a cross correlation centroid-finding algorithm to determine the best match of the particle/cell position in successive images. With routine distance calibration, the overlay of the image stack provided a quantitative assessment of the particle/cell path.
Speckle Displacement Velocimetry
After routine distance calibration, the recorded image stack was processed using a custom MatLab (MathWorks, Natick, MA) algorithm. The speckles are identified as high contrast regions with speckle position determined with subpixel accuracy. The speckle displacement was identified using a cross correlation algorithm to determine the best match of the speckle pattern displacement in successive images. Instantaneous velocity was calculated and plotted based on the time intervals based on the 100 mhz system bus clock of the Xeon processor (Intel, Santa Clara, CA).
Time-Series Flow Visualization
The stream acquired images were stacked to create a time-series of 500 or 1000 consecutive frames. The stacks were systematically analyzed to ensure the absence of motion artifact. The stack “maximum” operation selected the highest intensity value for each pixel location throughout the time-series. The resultant image, reflecting a time-series reconstruction of particle locations during the time interval of the image stack, was segmented by particle density and pseudocolored into “high flow” and “low flow” regions. The pseudocolored image was overlaid on the original video series to facilitate the analysis of flow distribution.
After euthanasia, subtotal colectomy (cecum to sigmoid) was performed. The lumen was flushed and opened along the mesenteric border (McDonald and Newberry,2007). The mucosa was copiously irrigated with cold PBS (4°C) until all debris was removed as determined by stereomicroscopy. The colon wall was immobilized on a standard microscope slide and the mucosa, superficial to the lamina propria, was removed using gentle dissection with a second microscope slide. Limited dissection of the superficial (∼50 μm thick) mucosa was confirmed by light microscopy. The microdissected mucosa was used for all subsequent mRNA analyses.
Total RNA was isolated using Qiagen RNeasy Midi Kit (Qiagen, Valencia, CA). Briefly, the fresh tissue was homogenized using a rotor-stator homogenizer for 60s until uniformly homogeneous. The tissue lysate was centrifuged at 3,000g for 10 min and the supernatant (lysate) was removed by pipetting. An equal volume of 70% ethanol was added to lysate and gently mixed. The sample was placed in a RNeasy midi column, centrifuged for 5 min at 3,000g and the flow-trough was discarded. After additional RPE buffer was added to the column, the tube was again centrifuged for 5 min at 3000g to dry the RNeasy silica-gel membrane. The RNeasy column was transferred to a collection tube and elution was performed using RNase-free water and centrifugation for 3 min at 3,000g. Generally, a second elution step was not performed. Genomic DNA contamination was eliminated by RNase-Free DNase Set (Qiagen). Briefly, 1–2 ug of potentially contaminated RNA was treated with DNase buffer, RNase inhibitor and DNase I. In all RNA isolations, the total RNA quality was assessed by using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA integrity numbers (RIN) (Schroeder et al.,2006) of the RNA samples were uniformally greater than 7.3 (mean 8.5; range, 7.3–9.8).
First Strand cDNA Synthesis used RT2 First Strand Kit from SuperArray Bioscience Corporation. Mouse Angiogenesis RT2 Profiler PCR Array and RT2 Real-Timer SyBR Green/ROX PCR Mix were purchased from SuperArray Bioscience Corporation (Frederick, MD). The genes of interest in this study are shown in Table 1.
|Ccl3 (MIP-1a)||Chemokine (C-C motif) ligand 3||Al323804/G0S19-1|
|Ccl5 (RANTES)||Chemokine (C-C motif) ligand 5||MuRantes/RANTES|
|Ccl7 (MCP-3)||Chemokine (C-C motif) ligand 7||MCP-3/Scya7|
|Ccl17 (TARC)||Chemokine (C-C motif) ligand 17||ABCD-2/Scya17|
|Ccl22||Chemokine (C-C motif) ligand 22||ABCD-1/DCBCK|
|Cxcl1||Chemokine (C-X-C motif) ligand 1||Fsp/Gro1|
|Cxcl4 (Pf4)||Platelet factor 4||Cxcl4/Scyb4|
|Cxcl5 (ENA-78)||Chemokine (C-X-C motif) ligand 5||AMCF-II/ENA-78|
|Cxcl12||Chemokine (C-X-C motif) ligand 12||Al174028/PBSF|
|Ccr 1||Chemokine (C-C motif) receptor 1||Cmkbr1/Mip-1a-R|
|Ccr 2||Chemokine (C-C motif) receptor 2||Cc-ckr-2/Ccr2a|
|Ccr 5||Chemokine (C-C motif) receptor 5||AM4-7/CD195|
|II8rb||Interleukin 8 receptor, beta||CD128/CDw128|
Real-time PCR was performed with SYBR green qPCR master mixes that include a chemically-modified hot start Taq DNA polymerase (SABioscience). PCR was performed on ABI 7300 Real-Time PCR System (Applied Biosystems). All reactions were 50 cycles using standard ABI cycling conditions (initial 2 minutes at 50°C, 10 minutes at 95°C and 1 minute annealing and extension at 60°C).
Gene expression was calculated using the comparative cycle threshold (Ct) method (Livak and Schmittgen,2001). Although the data was monitored for nonideal efficiencies, comparable amplification of the target genes and reference genes was assumed. Every effort to optimize the reaction efficiency was made. Validation assays using serial dilutions of the target and reference genes were not routinely performed. The DSS-induced colitis and control data were plotted as a scattergram and a linear regression was calculated with 95% prediction bands after the data was imported into Origin 8.0 (OriginLab, North Hampton, MA). Linear regression was uniformly P < 0.0001. In nanoparticle velocity analyses, the unpaired Student's t test for samples of unequal variances was used to calculate statistical significance. The data was expressed as mean ± one standard deviation. The significance level for the sample distribution was defined as P < 0.01. Volcano plots of the RT-PCR data were constructed with log fold change on the X-axis (reflecting biologic impact) and the p value on the Y-axis (statistical significance). The p values, representing the t test comparison of inflamed and control microdissected mucosa, were plotted on a negative log scale to facilitate presentation.
Flow Patterns in the Mucosal Plexus
The anatomy of the mucosal plexus, a quasi-polygonal network surrounding the mucosal crypts, was confirmed by corrosion casting and scanning electron microscopy (Fig. 1A,B) as well as epi-illumination intravital microscopy (Fig. 1C). In acute chemically-induced colitis, videomicroscopy of intravascular fluorescent tracers demonstrated flow velocities with wide dispersion (Fig. 1D). Despite a lower mean velocity in colitis mice, the variability in blood flow velocity was higher than in control mice. To provide a more quantitative assessment of flow changes, systematic and random sampling of the mucosal plexus flow paths was performed. Tracer flux, reflecting volumetric blood flow, confirmed the flow variation in the colitis mice (Fig. 2A,B). Analysis of variance demonstrated a highly significant difference between control and colitis animals (Fig. 2C). The tracer flux variability appeared to be spatially associated with areas of zero flow; that is, vessel segments in which tracer flow was excluded. The number of excluded segments was significantly higher in colitis animals (P < .01; Fig. 2D).
Stable Platelet Adhesions to the Endothelium
Microvessel segments excluded from mucosal plexus blood flow suggested a potential role for platelets in producing the observed blood flow patterns. After the intravascular injection of fluorescently labeled platelets, regions of tracer exclusion were spatially associated with the stable accumulation of platelets within the mucosal plexus. Within 1 hr of injection, stable or fixed platelet aggregates were present in 15% of excluded segments (N = 6; range, 12%–23%) (Fig. 3). The remaining excluded segments, not demonstrating platelet fluorescence, likely represented pre-existing thrombus (inaccessible to fluorescently labeled platelets) or alternative mechanisms of flow exclusion.
In contrast to platelet-associated occlusion of microvessels within the mucosal plexus, platelet aggregates were frequently observed at the bifurcations of collecting veins. The platelet aggregates demonstrated stable adherence to the endothelium, but dynamic extensions into the vessel lumen (Fig. 4A). The blood flow velocity in the collecting veins, generally higher than in the mucosal plexus, suggested that the fluctuating luminal aggregates were responding to flow variation (Fig. 4B). Regardless of the causal relationship, the apparent size of the aggregates varied over seconds (Fig. 4C) and was associated with platelet microaggregates in the flow stream (Fig. 4A, arrow).
Reversible Flow Exclusion and Rolling Aggregates
In many capillaries and collecting veins, platelets were observed rolling on the endothelium. Although occasional platelet microaggregates were seen in the collecting veins of control mice, larger aggregates were frequent in both the mucosal plexus and collecting veins of colitis mice. In six colitis mice (TNBS N = 3; DSS N = 3), the rolling velocity of platelet aggregates ranged from 14 to 186 μm/sec (mean = 113 μm/sec). Because the mucosal plexus is an interconnected network of microvessels, rolling platelet aggregates were associated with transient occlusion of the collecting veins. Transient occlusions were followed by redistribution of blood flow and bidirectional movement of the aggregates. This pattern of reversible flow exclusion is illustrated in Fig. 5A. As the platelet aggregate rolled “upstream,” connecting segments (Fig. 5A, b1, and b2) of the mucosal plexus were initially excluded from the flow stream. With sufficient upstream displacement of the aggregate, the tracers in segments b1 and b2 subsequently passed into the draining vein. The velocity of the tracers, reflected in the b1 and b2 flow paths, was temporally linked to platelet displacement (Fig. 5B). Of note, the rolling velocity of the platelet aggregate (50–100 μm/sec; Fig. 5C) was typical of both large and small platelet aggregates.
Platelet-Associated Gene Expression
The numerous platelet aggregates in the mucosal plexus suggested the importance of the local inflammatory mileau in platelet activation and subequent blood flow dynamics. To investigate the expression of platelet-associated factors associated with platelet activation, mRNA was harvested by microdissection of the mucosal plexus. Because of the more homogeneous and reproducible response to DSS, the longitudinal study of platelet-associated gene expression was limited to DSS-induced colitis. RT-PCR array analysis of gene expression was studied at 7, 14, 31, and 65 days after the start of DSS exposure. The peak of chemokine expression occurred 14 days after the start of DSS (Fig. 6A). Consistent with intense inflammation, expression of genes for CCL3, CXCL1, CCL2, CXCL5, CCL7, CCL8, and Il-1b were significantly increased (P < 0.01), whereas the expression of CCL6 was significantly decreased (P < 0.01) 14 days after the onset of inflammation (Fig. 6B).
In this report, we quantitatively assessed mucosal blood flow dynamics in chemically-induced murine colitis. The blood flow patterns within the mucosal plexus demonstrated (1) wide dispersion of flow velocity, (2) microvessel segments excluded from blood flow, (3) platelet aggregates spatially associated with blood flow perturbations, and (4) enhanced expression of platelet agonists within the mucosal plexus. These results suggest that platelet aggregation, activated by the inflammatory mileau, is a primary mechanism responsible for the complex flow dynamics observed in acute murine colitis.
Our in vivo observations suggest that the variability in mucosal plexus blood flow is a reflection of both platelet aggregation and the underlying structural anatomy of the plexus. The mucosal microcirculation is a nonparallel arteriovenous system fed by one or two central feeding arteries and draining marginal veins defining ∼6500 um2 of the mucosal plexus (Turhan et al.,2007). Within these flow regions, the mucosal plexus is a densely interconnected quasi-polygonal network without evidence of precapillary sphincter-like activity (Turhan et al.,2007). As result of the network structure, the isolated occlusion of a plexus vessel segment results in little risk to tissue viability. Blood flow can rapidly adapt and redistribute to meet tissue demands. In the present study, these adaptive flow changes were observed in the vessels adjacent to the occluded segment. In most instances, there was a sharp increase in blood flow in the segments connected to areas of presumed platelet-associated occlusion. The rapid adaptive changes in blood flow were even more apparent as a result of rolling platelet aggregates. Reversible occlusions were associated with rapid changes in mucosal plexus flow velocity and the redistribution of blood flow.
The variability in blood flow was spatially associated with microvascular segments that excluded intravascular tracers. In this report, we identified two mechanisms of flow exclusion. First, stable or fixed aggregates were identified within mucosal plexus capillaries. Second, rolling platelet aggregates in the collecting veins of the mucosal plexus resulted in reversible obstruction of segments of the mucosal plexus. Although platelets were spatially associated with perturbed blood flow patterns, it is likely that leukocytes and the coagulation cascade participated as well. Patients with inflammatory bowel disease have been shown to have more platelet-leukocyte aggregates (PLA) than healthy or inflammatory control subjects (Irving et al.,2008). It is possible that the microaggregates observed in the draining veins represented both platelet aggregates as well as PLA. Similarly, several studies have indicated subclinical activation of the coagulatory cascade (Chamouard et al.,1995; Souto et al.,1995). The vascular segments that excluded blood flow tracers likely reflected activation of both the coagulation cascade and circulating platelets.
Dynamic changes in blood patterns have adaptive consequences beyond sustaining organ viability and maintaining tissue function. Growing evidence indicates that endothelial cells respond to mechanical factors such as fluid shear stress (Davies,1995; Gimbrone et al.,2000; Chien,2007). Wall shear stress, reflecting both flow velocity and vessel geometry, activates a variety of mechanosensors (Labrador et al.,2003; Chien,2007). Membrane molecules include receptor tyrosine kinases (Chen et al.,1999; Wang et al.,2002), integrins (Jalali et al.,2001; Schwartz,2001), ion channels (Olesen et al.,1988; Yamamoto et al.,2006), G protein receptors (Kuchan et al.,1994) and even lipids (Haidekker et al.,2000). In turn, these membrane mechanoreceptors trigger signaling pathways that activate multiple genes that can be both homeostatic and proinflammatory (Chien,2007). Here, we show that platelet aggregates in acute colitis produce abrupt changes in mucosal plexus blood flow. Additional work will be needed to determine if these changes in blood flow trigger the gene transcription associated with the structural adaptations and proinflammatory consequences observed in chronic colitis.
Whereas the presence of stable platelet aggregates within the mucosal plexus was consistent with the presence of strong agonists such as collagen and thrombin, the prevalence of dynamic or metastable aggregates suggested the presence of so-called weak agonists. Weak agonists have recently been shown to include a variety of chemokines including CCL5, CCL17, and CXCL12 (Clemetson et al.,2000; Abi-Younes et al.,2001; Shenkman et al.,2004). In our colitis model, we investigated the presence of these weak agonists by characterizing gene expression within the microdissected mucosal plexus. We analyzed platelet gene pathways of both blood vessels and infiltrating inflammatory cells. The enhanced expression of multiple platelet agonists, including CCL3, CCL5, CCL7, CXCL1, and CXCL5 indicates the prominent expression of multiple platelet agonists within the inflammatory microenvironment.
An intriguing observation is the significant elevation in CXCL1 gene expression in our model of murine colitis. Originally identified by subtractive hybridization of tumorigenic cells (Anisowicz et al.,1987), the chemokine CXCL1 is a high affinity ligand of the CXCR2 receptor and structurally related to CXCL5 and IL-8. CXCL1 is a potent mediator of tumor-associated angiogenesis (Strieter et al.,1995; Wang et al.,2006) and leukocyte chemoattraction. CXCL1 is found in platelet alpha-granules along with a number of other chemokines capable of attracting leukocytes and further activating other platelets (Gear and Camerini,2003). Several findings have linked CXCL1 to colonic disease. Enhanced CXCL1 expression in the colon has been demonstrated in a variety of neoplastic conditions (Rubie et al.,2008) as well as in response to injury and/or inflammatory cytokines (Yang et al.,1997; Song et al.,1999; Thorpe et al.,2001). In addition to other studies demonstrating enhanced mRNA expression (Qualls et al.,2006; Wu and Chakravarti,2007), increased levels of the CXCL1 chemokine have been demonstrated in the plasma of mice with chemically-induced colitis (Karlsson et al.,2008) as well as in the bowel lumen of human patients with colitis (Egesten et al.,2007). Finally, mice with a genetically engineered deficiency of CXCL1 have a markedly dysregulated response to DSS (Shea-Donohue et al.,2008). Although these results suggest an important role for CXCL1 in both human and murine colitis, the coexpression of multiple other platelet agonists suggest that single variable manipulations such as treatment with a single chemokine antagonist are unlikely to be revealing. The complexity of the inflammatory mileau will require an integrated experimental approach that assesses both extravascular inflammation and intravascular blood flow.
Finally, this study illustrates the challenges of characterizing the complex adaptive responses of the inflammatory microcirculation. In the mucosal plexus, segmental vascular occlusions were associated with not only variation in blood flow velocity, but also changes in blood flow direction. The complex blood flow patterns were not easily characterized by studying isolated components of the system. Future studies will benefit from (1) the development of spatial statistics capable of characterizing the variable velocity profiles across than network, (2) mosaicized video recordings that provide a near real-time assessment of blood flow patterns within entire flow regions of the mucosal plexus, and (3) network computational models that can accurately simulate perturbations in system components. These developments would provide useful insights into not only murine colitis, but similar biological processes characterized by adaptive change and feedback control.
- 2001. The CC chemokines MDC and TARC induce platelet activation via CCR4. Thromb Res 101: 279–289. , , .
- 1987. Constitutive overexpression of a growth-regulated gene in transformed chinese-hamster and human cells. Proc Natl Acad Sci USA 84: 7188–7192. , , .
- 2006. Mechanisms underlying the anti-inflammatory actions of boswellic acid derivatives in experimental colitis. Am J Physiol Gastrointestinal Liver Physiol 290: G1131–G1137. , , , , , , , , , , , .
- 2004. Tracking of leukocyte recruitment into tissues of mice by in situ labeling of blood cells with the fluorescent dye CFDA SE. J Immunol Methods 286: 69–78. , , , .
- 1995. Prothrombin fragment 1 + 2 and thrombin-antithrombin III complex as markers of activation of blood coagulation in inflammatory bowel diseases. Eur J Gastroenterol Hepatol 7: 1183–1188. , , , , , , , .
- 1999. Mechanotransduction in response to shear stress. Roles of receptor tyrosine kinases, integrins, and Shc. J Biol Chem 274: 18393–18400. , , , , , , .
- 2007. Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell. Am J Physiol Heart Circ Physiol 292: H1209–H1224. .
- 2000. Functional expression of CCR1, CCR3, CCR4, and CXCR4 chemokine receptors on human platelets. Blood 96: 4046–4054. , , , , , .
- 2003. Platelets trigger a CD40-dependent inflammatory response in the microvasculature of inflammatory bowel disease patients. Gastroenterology 124: 1249–1264. , , , , , , , .
- 1995. Flow-mediated endothelial mechanotransduction. Physiol Rev 75: 519–560. .
- 1992. Mucosal capillary thrombi in rectal biopsies. Histopathology 21: 127–133. , , , , , , , .
- 1966. Early histological changes in ulcerative colitis. A light and electron microscopic study. Gastroenterology 50: 519–540. .
- 2007. The proinflammatory CXC-chemokines GRO-alpha/CXCL1 and MIG/CXCL9 are concomitantly expressed in ulcerative colitis and decrease during treatment with topical corticosteroids. Int J Colorectal Dis 22: 1421–1427. , , , , , , .
- 2003. Platelet chemokines and chemokine receptors: linking hemostasis, inflammation, and host defense. Microcirculation 10: 335–350. , .
- 2000. Endothelial dysfunction, hemodynamic forces, and atherogenesis. N Y Acad Sci 902: 230–240. , , , , .
- 2000. Fluid shear stress increases membrane fluidity in endothelial cells: a study with DCVJ fluorescence. Am J Physiol Heart Circ Physiol 278: H1401–1406. , , .
- 1993. Factor XIIIA subunit and Crohn's disease. Gut 34: 75–79. , , , , , , , .
- 2008. Platelet-leucocyte aggregates form in the mesenteric vasculature in patients with ulcerative colitis. Eur J Gastroenterol Hepatol 283–289. , , , , , , , , .
- 2001. Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands. Proc Natl Acad Sci USA 98: 1042–1046. , , , , , , , .
- 2008. Intra-colonic administration of the TLR7 agonist R-848 induces an acute local and systemic inflammation in mice. Biochem Biophys Res Commun 367: 242–248. , , , , , , , , , .
- 1998. Impact of fibroblast growth factor-2 on tumor microvascular architecture. A tridimensional morphometric study. Am J Pathol 152: 1607–1616. , , , , , , , .
- 2001. 3D microvascular architecture of pre-cancerous lesions and invasive carcinomas of the colon. Br J Cancer 84: 1354–1362. , , .
- 1994. Role of G proteins in shear stress-mediated nitric oxide production by endothelial cells. Am J Physiol 267: C753–C758. , , .
- 2003. Interactions of mechanotransduction pathways. Biorheology 40: 47–52. , , , , , .
- 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402–408. , .
- 2007. Whole-mount techniques to evaluate subepithelial cellular populations in the adult mouse intestine. Biotechniques 43: 50–56. , .
- 2005a. Molecular determinants of the prothrombogenic phenotype assumed by inflamed colonic venules. Am J Physiol Gastrointest Liver Physiol 288: G920–G926. , , , , , .
- 2005b. Colonic blood flow responses in experimental colitis: time course and underlying mechanisms. Am J Physiol Gastrointest Liver Physiol 289: G1024– G1029. , , , , , , , , .
- 1990. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology 98: 694–702. , , , , , .
- 1988. Haemodynamic shear stress activates a K+ current in vascular endothelial cells. Nature 331: 168–170. , , .
- 2006. Suppression of experimental colitis by intestinal mononuclear phagocytes. J Leukoc Biol 80: 802–815. , , , .
- 2005. Vessel painting of the microcirculation using fluorescent lipophilic tracers Microvasc Res 70: 90–96. , , , , , , .
- 2007a. Murine microvideo endoscopy of the colonic microcirculation. J Surg Res 142: 97–103. , , , , , .
- 2007b. Structural adaptations in the murine colon microcirculation associated with hapten-induced inflammation. Gut 56: 518–523. , , , , , , .
- 2006a. Multi-frame particle tracking in intravital imaging: defining lagrangian coordinates in the microcirculation. BioTechniques 41: 597–601. , , , , , , .
- 2006b. Multi-image particle tracking velocimetry of the microcirculation using fluorescent nanoparticles. Microvasc Res 72: 27–33. , , , , , .
- 2007. PECAM-1 (CD 31) mediates transendothelial leukocyte migration in experimental colitis. Am J Physiol Gastrointest Liver Physiol 293: G446–G452. , , , , , , , , .
- 2006. Critical role of endothelial P-selectin glycoprotein ligand 1 in chronic murine ileitis. J Exp Med 203: 907–917. , , , , , , .
- 2008. ELR plus CXC chemokine expression in benign and malignant colorectal conditions. BMC Cancer 8: 11. , , , , , , , .
- 2006. The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol 7: 3. , , , , , , , , , .
- 2001. Integrin signaling revisited. Trends Cell Biol 11: 466–470. .
- 2008. Mice deficient in the CXCR2 ligand, CXCL1 (KC/GRO-alpha), exhibit increased susceptibility to dextran sodium sulfate (DSS)-induced colitis. Innate Immunity 14: 117–124. , , , , , , , , .
- 2004. Differential response of platelets to chemokines: RANTES non-competitively inhibits stimulatory effect of SDF-1 alpha. J Thromb Haemost 2: 154–160. , , , , , .
- 1999. Expression of the neutrophil chemokine KC in the colon of mice with enterocolitis and by intestinal epithelial cell lines: Effects of flora and proinflammatory cytokines. J Immun 162: 2275–2280. , , , , , , , , , .
- 1995. Prothrombotic state and signs of endothelial lesion in plasma of patients with inflammatory bowel disease. Dig Dis Sci 40: 1883–1889. , , , , , , .
- 1995. The functional role of the ELR motif in CXC chemokine-mediated angiogenesis. J Biol Chem 270: 27348–27357. , , , , , , , , , , , , .
- 2001. Shiga toxins induce, superinduce, and stabilize a variety of C-X-C chemokine mRNAs in intestinal epithelial cells, resulting in increased chemokine expression. Infect Immun 69: 6140–6147. , , , .
- Bimodal oscillation frequencies of blood flow in the inflammatory colon microcirculation. Anat Rec 292: 65–72. , , , , , , .
- 2007. Bridging mucosal vessels associated with rhythmically oscillating blood flow in murine colitis. Anat Rec 291: 74–92. , , , , , , .
- 2007a. CD40-CD40 ligand mediates the recruitment of leukocytes and platelets in the inflamed murine colon. Gastroenterology 132: 955–965. , , , , , , , , , , , .
- 2007b. Mechanisms of platelet and leukocyte recruitment in experimental colitis. Am J Physiol Gastrointest Liver Physiol 293: G1054–G1060. , , , , , , , , .
- 1989. Pathogenesis of Crohn's disease: multifocal gastrointestinal infarction. Lancet 2: 1057–1062. , , , , , , .
- 2006. CXCL1 induced by prostaglandin E-2 promotes angiogenesis in colorectal cancer. J Exp Med 203: 941–951. , , , , , , , , , .
- 2002. Interplay between integrins and FLK-1 in shear stress-induced signaling. Am J Physiol Cell Physiol 283: C1540–1547. , , , , , , , .
- 2007. Differential expression of inflammatory and fibrogenic genes and their regulation by NF-kappa B inhibition in a mouse model of chronic colitis. J Immunol 179: 6988–7000. , .
- 2006. Impaired flow-dependent control of vascular tone and remodeling in P2X4-deficient mice. Nat Med 12: 133–137. , , , , , , , , , , , , , , , , .
- 1997. Differential and regulated expression of C-X-C, C-C, and C-chemokines by human colon epithelial cells. Gastroenterology 113: 1214–1223. , , , .
- 2002. Improved significance test for DNA microarray data: temporal effects of shear stress on endothelial genes. Physiol Genomics 12: 1–11. , , , , , , , .