Distribution of Amelotin in Mouse Tooth Development

Authors

  • Yuguang Gao,

    Corresponding author
    1. Department of Pediatric and Preventive Dentistry, Institute of Stomatology, Weifang Medical University, Weifang, Shandong Province, China
    • Department of Pediatric and Preventive Dentistry, Institute of Stomatology, Weifang Medical University, 288 Shengli East Street, Kuiwen District, Weifang City, Shandong Province 261042, China
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    • Fax: 86-536-260-2994

  • Wanchun Wang,

    1. Department of Oral Health Care, Qingdao Hospital of Stomatology, Qingdao, Shandong Province, China
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  • Yan Sun,

    1. Department of Pediatric and Preventive Dentistry, Institute of Stomatology, Weifang Medical University, Weifang, Shandong Province, China
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  • Juanjuan Zhang,

    1. Department of Pediatric and Preventive Dentistry, Institute of Stomatology, Weifang Medical University, Weifang, Shandong Province, China
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  • Dongliang Li,

    1. Department of Pediatric and Preventive Dentistry, Institute of Stomatology, Weifang Medical University, Weifang, Shandong Province, China
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  • Yahong Wei,

    1. Department of Pediatric and Preventive Dentistry, Institute of Stomatology, Weifang Medical University, Weifang, Shandong Province, China
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  • Tingting Han

    1. Department of Pediatric and Preventive Dentistry, Institute of Stomatology, Weifang Medical University, Weifang, Shandong Province, China
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Abstract

Amelotin is expressed and secreted by ameloblasts in tooth development, but amelotin distribution during enamel development is not clear. In this report, we first investigated amelotin expression in developing teeth by immunohistochemistry. Amelotin was detected in the enamel matrix at the secretion and maturation stages of enamel development. Amelotin was also observed at Tomes' processes on the apical ends of secretory ameloblasts. We then compared amelotin gene expression with those of amelogenin, enamelin, and ameloblastin in the mandibles of postnatal mice by RT-PCR. The expression of amelotin was detected as early as in postnatal day 0 mandibles and amelotin was coexpressed with amelogenin, ameloblastin, and enamelin during tooth development. These data strongly suggest that amelotin is an enamel matrix protein expressed at the secretion and maturation stages of enamel development. Anat Rec, 2010. © 2009 Wiley-Liss, Inc.

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