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Molecular Morphology of the Chick Heart Visualized by MALDI Imaging Mass Spectrometry
Article first published online: 23 FEB 2010
Copyright © 2010 Wiley-Liss, Inc.
The Anatomical Record
Volume 293, Issue 5, pages 821–828, May 2010
How to Cite
Grey, A. C., Gelasco, A. K., Section, J., Moreno-Rodriguez, R. A., Krug, E. L. and Schey, K. L. (2010), Molecular Morphology of the Chick Heart Visualized by MALDI Imaging Mass Spectrometry. Anat Rec, 293: 821–828. doi: 10.1002/ar.21103
- Issue published online: 23 APR 2010
- Article first published online: 23 FEB 2010
- Manuscript Accepted: 9 NOV 2009
- Manuscript Received: 3 JUL 2008
- National Institutes of Health. Grant Number: C06 RR018823
- NIH NHLBI Cardiovascular Proteomics Center. Grant Number: N01-HV-28181
- NIH Grant (A Summer Undergraduate Research Program Grant). Grant Number: T35HL007769
- Extramural Research Facilities Program of the National Center for Research Resources
Additional Supporting Information may be found in the online version of this article.
|AR_21103_sm_suppinfofig1.tif||2175K||Supporting Figure S1: Comparison of tissue morphology from frozen and acid/ethanol fixed tissue. Optical images of acid/ethanol fixed (Left panel) and frozen (Right panel) chick heart sections. Fine tissue structures are apparent in the fixed tissue section, but absent in the frozen tissue section. Note that upper left portion of extracardiac tissue was removed during dissection of fixed tissue sample.|
|AR_21103_sm_suppinfofig2.tif||16664K||Supporting Figure S2: Comparison of spectra from frozen and acid/ethanol fixed tissue. (A) Example spectrum from a chick heart section collected from frozen tissue, washed with ethanol and spotted with DHB matrix. (B) A higher number of spectral features, particularly in the low mass range, and increased total ion current are evident in an example spectrum from an acid fixed/paraffin embedded chick heart tissue section prepared with DHB matrix. Although ethanol washes could remove hydrophilic proteins from frozen sections, this step is commonly used to produce optimum matrix crystallization and the highest quality images from frozen tissue. Both spectra are plotted on the same absolute intensity scale.|
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