Effect of Hypoxia/Reoxygenation on Cell Viability and Expression and Secretion of Neurotrophic Factors (NTFs) in Primary Cultured Schwann Cells

Authors

  • Hao Zhu,

    1. Department of Anatomy, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University Institutes of Medical Sciences, Shanghai, China
    Search for more papers by this author
    • 1

      Authors contributed equally to this work.

  • Feng Li,

    1. Department of Anatomy, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University Institutes of Medical Sciences, Shanghai, China
    Search for more papers by this author
    • 1

      Authors contributed equally to this work.

  • Wen-Juan Yu,

    1. Department of Psychiatry, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Search for more papers by this author
  • Wen-Jin WANG,

    1. Department of Anatomy, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University Institutes of Medical Sciences, Shanghai, China
    Search for more papers by this author
  • Liu Li,

    1. Department of Anatomy, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University Institutes of Medical Sciences, Shanghai, China
    Search for more papers by this author
  • Li-Dan Wan,

    1. Department of Anatomy, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University Institutes of Medical Sciences, Shanghai, China
    Search for more papers by this author
  • Yan Le,

    1. Department of Anatomy, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University Institutes of Medical Sciences, Shanghai, China
    Search for more papers by this author
  • Wen-Long Ding

    Corresponding author
    1. Department of Anatomy, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University Institutes of Medical Sciences, Shanghai, China
    • Department of Anatomy, Shanghai Jiao Tong University School of Medicine, No. 227 South Chongqing Road, Shanghai 200025, China
    Search for more papers by this author

Abstract

As the primary myelin-forming cells of the peripheral nervous system, Schwann cells (SCs) play a key role in the regeneration of injured peripheral nerves. However, hypoxia causes injury of SCs, as observed in peripheral neuropathies, including those caused by diabetes. So we investigated the effect of hypoxia/reoxygenation (H/R) on SCs in this study. To do so, SCs were cultured in hypoxic condition in vitro and then in normal condition for 24 hr; The effects H/R on SCs were evaluated by MTT (3(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) assay, Hoechst staining, immunocytochemistry, western blotting, ELISA, and RT-PCR. H/R resulted in a significant decrease in SCs survival and an increase in caspase-3 activity. H/R also reduced the mRNA level of BDNF (brain derived neurotrophic factor) and its secretion, but NGF mRNA level was elevated in these cells. These observations showed that H/R induces death of primary cultured SCs, and different mechanisms responsible for regulating NGF and BDNF expression. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.

Ancillary