Lei Jianga and Yangchao Chen contributed equally to this work.
Dynamic Transcriptional Changes of TIEG1 and TIEG2 During Mouse Tissue Development
Article first published online: 3 MAR 2010
Copyright © 2010 Wiley-Liss, Inc.
The Anatomical Record
Volume 293, Issue 5, pages 858–864, May 2010
How to Cite
Jiang, L., Chen, Y., Chan, C.-Y., Lu, G., Wang, H., Li, J.-C. and Kung, H.-F. (2010), Dynamic Transcriptional Changes of TIEG1 and TIEG2 During Mouse Tissue Development. Anat Rec, 293: 858–864. doi: 10.1002/ar.21108
- Issue published online: 23 APR 2010
- Article first published online: 3 MAR 2010
- Manuscript Accepted: 1 DEC 2009
- Manuscript Received: 5 NOV 2008
- Hong Kong Research Grants Council (GRF Grant). Grant Numbers: CUHK462109, CUHK7422/03M, 467507
- Special Grant of the Major State Basic Research Program of China. Grant Number: 2006CB910100
- Foundation of Guangzhou Science and Technology Bureau. Grant Number: 2005Z1-E013
- Chinese University of Hong Kong
- Li Ka Shing Institute of Health Sciences
- mRNA expression
TGF-β-inducible early-response gene (TIEG) is a family of primary response genes induced by TGF-β, which are well recognized in regulating cellular proliferation and apoptosis. However, their expression profile has never been investigated during embryogenesis in different organs. In this study, we aimed to investigate the transcriptional level of both TIEG1 and TIEG2 during development in various mice organs, including the brain cortex, cerebellum and stem, brain striatum, muscle, heart, liver, kidney, and lung. Quantitative real-time PCR was used to profile the change of transcriptional level of the two TIEG members in the mice tissues at six developmental stages. Taken together, the expression of TIEG1 and TIEG2 was specific in different organs yet varied with different developmental time points. Their dynamic changes were moderately consistent in most organs including the brain cortex, striatum, liver, kidney, and lung. However, their mRNA expression in both the heart and muscle was significantly different at all developmental stages, which might propose a compensation of functions in the TIEG family. Nevertheless, our data indicate that both the TIEG genes are essential in regulating the normal organ development and functioning in murine model, as their expressions were ubiquitous and tissue specific at various developmental stages. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.