Dynamic Transcriptional Changes of TIEG1 and TIEG2 During Mouse Tissue Development

Authors

  • Lei Jiang,

    1. Institute of Cell Biology, Zhejiang University, Hangzhou, People's Republic of China
    2. Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin, HKSAR
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    • Lei Jianga and Yangchao Chen contributed equally to this work.

  • Yangchao Chen,

    1. Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, HKSAR
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    • Lei Jianga and Yangchao Chen contributed equally to this work.

  • Chu-Yan Chan,

    1. Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin, HKSAR
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  • Gang Lu,

    1. Department of Surgery, The Chinese University of Hong Kong, Shatin, HKSAR
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  • Hua Wang,

    1. Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin, HKSAR
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  • Ji-Cheng Li,

    Corresponding author
    1. Institute of Cell Biology, Zhejiang University, Hangzhou, People's Republic of China
    • Institute of Cell Biology, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China
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    • Phone: +86-571-88208088; Fax: +86-571-88208094

  • Hsiang-Fu Kung

    Corresponding author
    1. Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin, HKSAR
    • Stanley Ho Centre for Emerging Infectious Diseases, Rm511A, Basic Medical Sciences Building, The Chinese University of Hong Kong, Shatin, HKSAR
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    • Phone: +852-2603-7743; Fax: +852-2994-4988


Abstract

TGF-β-inducible early-response gene (TIEG) is a family of primary response genes induced by TGF-β, which are well recognized in regulating cellular proliferation and apoptosis. However, their expression profile has never been investigated during embryogenesis in different organs. In this study, we aimed to investigate the transcriptional level of both TIEG1 and TIEG2 during development in various mice organs, including the brain cortex, cerebellum and stem, brain striatum, muscle, heart, liver, kidney, and lung. Quantitative real-time PCR was used to profile the change of transcriptional level of the two TIEG members in the mice tissues at six developmental stages. Taken together, the expression of TIEG1 and TIEG2 was specific in different organs yet varied with different developmental time points. Their dynamic changes were moderately consistent in most organs including the brain cortex, striatum, liver, kidney, and lung. However, their mRNA expression in both the heart and muscle was significantly different at all developmental stages, which might propose a compensation of functions in the TIEG family. Nevertheless, our data indicate that both the TIEG genes are essential in regulating the normal organ development and functioning in murine model, as their expressions were ubiquitous and tissue specific at various developmental stages. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.

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