BMP-2 Regulates the Formation of Oral Sulcus in Mouse Tongue by Altering the Balance Between TIMP-1 and MMP-13
Article first published online: 14 MAY 2010
Copyright © 2010 Wiley-Liss, Inc.
The Anatomical Record
Volume 293, Issue 8, pages 1408–1415, August 2010
How to Cite
Fukui, T., Suga, T., Iida, R.-H., Morito, M., Luan, X., Diekwisch, T. G.H., Nakamura, Y. and Yamane, A. (2010), BMP-2 Regulates the Formation of Oral Sulcus in Mouse Tongue by Altering the Balance Between TIMP-1 and MMP-13. Anat Rec, 293: 1408–1415. doi: 10.1002/ar.21164
- Issue published online: 22 JUL 2010
- Article first published online: 14 MAY 2010
- Manuscript Accepted: 18 FEB 2010
- Manuscript Received: 5 JUL 2009
- Ministry of Education, Culture, Sports, Science and Technology of Japan. Grant Numbers: 18592255, 19390501, 20592190
- oral sulcus;
The aim of this study is to investigate whether BMP-2 regulates the oral sulcus formation of mouse embryonic tongue by modifying the expression of TIMP and MMP. The BMP-2 siRNA induced a 180% increase in the depth of oral sulcus cavity (P < 0.01) by stimulating the invagination of oral sulcus into the mesenchymal tissues consisting of tongue floor, whereas the recombinant BMP-2 suppressed the process in the organ culture system of mouse embryonic tongue. The BMP-2 siRNA induced a 60% decrease in the expression of TIMP-1 mRNA (P < 0.05) and a drastic decline in TIMP-1 protein was observed around the oral sulcus in the BMP-2 siRNA treated mandibles. The recombinant BMP-2 induced a 220% increases in the expression of TIMP-1 mRNA and the area of the immunostaining for TIMP-1 around the oral sulcus was larger in the mandibles treated with the recombinant BMP-2 than the vehicle. The BMP-2 siRNA induced a 60% increase in the expression of MMP-13 protein and a marked increase in the staining intensity for MMP-13 was observed in the epithelial region of the BMP-2 siRNA treated mandibles. The recombinant BMP-2 induced a 70% decrease in the expression of MMP-13 mRNA and the decrease was mainly observed in the tissues around oral sulcus. The expressions of BMP-2, TIMP-1, and MMP-13 were verified in the tissues around in vivo developing oral sulcus at E11, 12, and 13 by immunohistochemistry. These results suggest that BMP-2 regulates the formation of oral sulcus by altering the balance between TIMP-1 and MMP-13. Anat Rec 293:1408–1415, 2010. © 2010 Wiley-Liss, Inc.