Ontogeny of the Kidney and Renal Developmental Markers in the Rhesus Monkey (Macaca Mulatta)
Article first published online: 3 SEP 2010
Copyright © 2010 Wiley-Liss, Inc.
The Anatomical Record
Volume 293, Issue 11, pages 1971–1983, November 2010
How to Cite
Batchelder, C. A., Lee, C. C. I., Martinez, M. L. and Tarantal, A. F. (2010), Ontogeny of the Kidney and Renal Developmental Markers in the Rhesus Monkey (Macaca Mulatta). Anat Rec, 293: 1971–1983. doi: 10.1002/ar.21242
- Issue published online: 29 OCT 2010
- Article first published online: 3 SEP 2010
- Manuscript Accepted: 21 JUN 2010
- Manuscript Received: 28 SEP 2009
- Center of Excellence in Translational Human Stem Cell Research (NIH). Grant Number: HL069748
- Primate Center base operating grant. Grant Number: RR00169
- The California Institute for Regenerative Medicine (CIRM). Grant Number: RC1-00144
- UC Davis Stem Cell Training Program. Grant Number: T1-00006
Nonhuman primates share many developmental similarities with humans, thus they provide an important preclinical model for understanding the ontogeny of biomarkers of kidney development and assessing new cell-based therapies to treat human disease. To identify morphological and developmental changes in protein and RNA expression patterns during nephrogenesis, immunohistochemistry and quantitative real-time PCR were used to assess temporal and spatial expression of WT1, Pax2, Nestin, Synaptopodin, alpha-smooth muscle actin (α-SMA), CD31, vascular endothelial growth factor (VEGF), and Gremlin. Pax2 was expressed in the condensed mesenchyme surrounding the ureteric bud and in the early renal vesicle. WT1 and Nestin were diffusely expressed in the metanephric mesenchyme, and expression increased as the Pax2-positive condensed mesenchyme differentiated. The inner cleft of the tail of the S-shaped body contained the podocyte progenitors (visceral epithelium) that were shown to express Pax2, Nestin, and WT1 in the early second trimester. With maturation of the kidney, Pax2 expression diminished in these structures, but was retained in cells of the parietal epithelium, and as WT1 expression was upregulated. Mature podocytes expressing WT1, Nestin, and Synaptopodin were observed from the mid-third trimester through adulthood. The developing glomerulus was positive for α-SMA (vascular smooth muscle) and Gremlin (mesangial cells), CD31 (glomerular endothelium), and VEGF (endothelium), and showed loss of expression of these markers as glomerular maturation was completed. These data form the basis for understanding nephrogenesis in the rhesus monkey and will be useful in translational studies that focus on embryonic stem and other progenitor cell populations for renal tissue engineering and repair. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.