MicroRNA-206 Is Associated With Invasion and Metastasis of Lung Cancer

Authors

  • Xiaochen Wang,

    1. Department of Thoraco-Cardiac Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China
    2. Department of Thoraco-Cardiac Surgery, Jiangyin First Hospital, Jiangyin, China
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    • Xiaochen Wang and Chunhua Ling contributed equally to this work.

  • Chunhua Ling,

    1. Department of Respiratory, The First Affiliated Hospital of Soochow University, Suzhou, China
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    • Xiaochen Wang and Chunhua Ling contributed equally to this work.

  • Yanyan Bai,

    1. Department of Neurology, The First Affiliated Hospital of Soochow University, Suzhou, China
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  • Jun Zhao

    Corresponding author
    1. Department of Thoraco-Cardiac Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China
    2. Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China
    • Department of Thoraco-Cardiac Surgery, The First Affiliated Hospital of Soochow University, 96 Shizi Street, Suzhou 215006, People's Republic of China
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Abstract

MicroRNAs are novel small noncoding RNA molecules that regulate gene expression at the post-transcriptional level. Compelling evidence reveals that there is a causative link between microRNAs deregulation and cancer development and progression. The present study aims to explore the function of miR-206 in the proliferation, apoptosis, motility, and invasion of nonsmall cell lung cancer. Using real-time PCR, we detected the miR-206 expression of normal lung tissues, tumor tissues, human normal bronchial epithelial cell line, and six lung cancer cell lines (LCCLs). Then, we evaluated the role of miR-206 in cell proliferation, apoptosis, and invasion using Cell Counting Kit-8 assay, Annexin-V/FITC assay, wound healing, and Transwell assay in LCCLs. As a result, miR-206 expression level was lower in high metastasis tumors and 95D than low metastasis tumors and normal lung tissues as well as other LCCLs. After miR-206 was upregulated in LCCLs, cell proliferation was notably attenuated and apoptosis was significantly increased. Furthermore, overexpression of miR-206 inhibited migration and invasion of lung cancer cells. In conclusion, our data suggest that expression level of miR-206 was inversely correlated with metastatic potential of lung cancer. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.

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