Oral Biology

A Morphological Analysis on the Osteocytic Lacunar Canalicular System in Bone Surrounding Dental Implants

  1. Maiko Haga1,2,†,*,
  2. Kayoko Nozawa-Inoue2,
  3. Minqi Li3,
  4. Kimimitsu Oda4,
  5. Sumio Yoshie1,
  6. Norio Amizuka3,
  7. Takeyasu Maeda2

Article first published online: 28 APR 2011

DOI: 10.1002/ar.21391

Issue

The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology

The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology

Volume 294, Issue 6, pages 1074–1082, June 2011

Additional Information

How to Cite

Haga, M., Nozawa-Inoue, K., Li, M., Oda, K., Yoshie, S., Amizuka, N. and Maeda, T. (2011), A Morphological Analysis on the Osteocytic Lacunar Canalicular System in Bone Surrounding Dental Implants. The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, 294: 1074–1082. doi: 10.1002/ar.21391

Author Information

  1. 1

    Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan

  2. 2

    Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

  3. 3

    Department of Development Biology of Hard Tissue, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan

  4. 4

    Division of Oral Biochemistry, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

  1. †

    Fax: +81-25-267-1134

*Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan

Publication History

  1. Issue published online: 20 MAY 2011
  2. Article first published online: 28 APR 2011

Funded by

  • MEXT (Ministry of Education, Culture, Sports, Science and Technology, Japan). Grant Number: 21791935

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Keywords:

  • osteocyte;
  • osteocytic lacunar canalicular system;
  • bone remodeling;
  • bone maturation;
  • titanium implantation;
  • tissue reaction

Abstract

Osseointegration is the most preferable interface of dental implants and newly formed bone. However, the cavity preparation for dental implants often gives rise to empty lacunae or pyknotic osteocytes in bone surrounding the dental implant. This study aimed to examine the chronological alternation of osteocytes in the bone surrounding the titanium implants using a rat model. The distribution of the osteocytic lacunar canalicular system (OLCS) in bone around the titanium implants was examined by silver impregnation according to Bodian's staining. We also performed double staining for alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), as well as immunohistochemistry for fibroblast growth factor (FGF) 23—a regulator for the serum concentration of phosphorus—and sclerostin, which has been shown to inhibit osteoblastic activities. Newly formed bone and the injured bone at the early stage exhibited an irregularly distributed OLCS and a few osteocytes positive for sclerostin or FGF23, therefore indicating immature bone. Osteocytes in the surrounding bone from Day 20 to Month 2 came to reveal an intense immunoreactivity for sclerostin. Later on, the physiological bone remodeling gradually replaced such immature bone into a compact profile bearing a regularly arranged OLCS. As the bone was remodeled, FGF23 immunoreactivity became more intense, but sclerostin became less so in the well-arranged OLCS. In summary, it seems likely that OLCS in the bone surrounding the dental implants is damaged by cavity formation, but later gradually recovers as bone remodeling takes place, ultimately inducing mature bone. Anat Rec,, 2011. © 2011 Wiley-Liss, Inc.

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