Immunohistochemical Analysis of the Proteolytic Enzyme Subtilase in the Digestive Organs of the Sea Urchin Strongylocentrotus intermedius

Authors

  • Kazuhiro Ura,

    Corresponding author
    1. Laboratory of Comparative Physiology, Graduate School of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan
    • Graduate School of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan
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  • Syuto Hasegawa,

    1. Laboratory of Comparative Physiology, Graduate School of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan
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  • Eri Tanaka,

    1. Laboratory of Comparative Physiology, Graduate School of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan
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  • Takahiro Gotoh,

    1. Laboratory of Comparative Physiology, Graduate School of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan
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  • Takao Ojima,

    1. Laboratory of Marine Molecular Biology, Graduate School of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan
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  • Yasuaki Takagi

    1. Laboratory of Comparative Physiology, Graduate School of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan
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Abstract

Subtilase, a major protease in the short-spined sea urchin (Strongylocentrotus intermedius), was isolated and used as antigen for the subsequent production of a specific polyclonal antibody. Immunoreactive cells were observed by immunohistochemical analysis in granules in the anterior and posterior stomach and the anterior intestine. These granules, which were most numerous in the anterior stomach, also stained intensely with methylene blue-Azure II. However, granules in cells of the esophagus, posterior intestine, and rectum were not stained by this antibody. We conclude that subtilase mainly localizes in the stomach and anterior intestine of the sea urchin. Anat Rec, 2012. © 2011 Wiley Periodicals, Inc.

Ancillary