Immunohistochemical Analysis of the Proteolytic Enzyme Subtilase in the Digestive Organs of the Sea Urchin Strongylocentrotus intermedius
Article first published online: 1 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
The Anatomical Record
Volume 295, Issue 1, pages 73–77, January 2012
How to Cite
Ura, K., Hasegawa, S., Tanaka, E., Gotoh, T., Ojima, T. and Takagi, Y. (2012), Immunohistochemical Analysis of the Proteolytic Enzyme Subtilase in the Digestive Organs of the Sea Urchin Strongylocentrotus intermedius. Anat Rec, 295: 73–77. doi: 10.1002/ar.21484
- Issue published online: 14 DEC 2011
- Article first published online: 1 OCT 2011
- Manuscript Accepted: 26 JUL 2011
- Manuscript Received: 6 APR 2011
- Ministry of Education, Science, and Culture, Japan
- sea urchin;
- digestive organ;
- granule cell;
Subtilase, a major protease in the short-spined sea urchin (Strongylocentrotus intermedius), was isolated and used as antigen for the subsequent production of a specific polyclonal antibody. Immunoreactive cells were observed by immunohistochemical analysis in granules in the anterior and posterior stomach and the anterior intestine. These granules, which were most numerous in the anterior stomach, also stained intensely with methylene blue-Azure II. However, granules in cells of the esophagus, posterior intestine, and rectum were not stained by this antibody. We conclude that subtilase mainly localizes in the stomach and anterior intestine of the sea urchin. Anat Rec, 2012. © 2011 Wiley Periodicals, Inc.