Laminar Expression of Ephrin-A2 in Primary Somatosensory Cortex of Postnatal Rats
Article first published online: 7 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
The Anatomical Record
Volume 295, Issue 1, pages 105–112, January 2012
How to Cite
Kenmuir, C. L., Chiaia, N. L., Lane, R. D. and Mooney, R. D. (2012), Laminar Expression of Ephrin-A2 in Primary Somatosensory Cortex of Postnatal Rats. Anat Rec, 295: 105–112. doi: 10.1002/ar.21485
- Issue published online: 14 DEC 2011
- Article first published online: 7 DEC 2011
- Manuscript Accepted: 18 AUG 2011
- Manuscript Received: 29 NOV 2010
- National Institutes of Health. Grant Number: P01-NS149048
Additional Supporting Information may be found in the online version of this article.
|AR_21485_sm_SuppFig1.tif||10431K||Supplementary Fig. 1. As a positive control for ephrin-A2 immunoreactivity, olfactory bulb sections were processed in parallel with cortical sections. In agreement with findings from other investigators in neonatal rat and mouse (references given in Materials and Methods section), ephrin-A2 protein is strongly expressed in the mitral cell layer (MCL) and in glomeruli (GL), and by P8, as shown here, had been largely downregulated in the primary afferent fibers (A, box denotes area shown at higher power in inset). As a negative control, olfactory bulbs, which were immunoreacted with the ephrin-A2 antibody adsorbed with its antigenic peptide, showed greatly reduced immunostaining (B). Scale bar is 100 µm for A and B and 33 µm for the insert in A.|
|AR_21485_sm_SuppFig2.tif||5013K||Supplementary Fig. 2. Confocal microscopic images of Hu and ephrin-A2 immunoreactivity in neurons in the border region between the supragranular layers above, where double labeled profiles are evident, and layer IV below, where few cell bodies or dendrites are double labeled.|
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