The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration
Article first published online: 18 NOV 2011
Copyright © 2011 Wiley-Liss, Inc.
The Anatomical Record
Volume 295, Issue 2, pages 234–248, February 2012
How to Cite
Lafontant, P. J., Burns, A. R., Grivas, J. A., Lesch, M. A., Lala, T. D., Reuter, S. P., Field, L. J. and Frounfelter, T. D. (2012), The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration. Anat Rec, 295: 234–248. doi: 10.1002/ar.21492
- Issue published online: 11 JAN 2012
- Article first published online: 18 NOV 2011
- Manuscript Accepted: 24 AUG 2011
- Manuscript Received: 22 MAY 2011
- NIH. Grant Numbers: EY017120, EY007551
- DePauw University's Professional Development Funds
- giant danio;
The paucity of mammalian adult cardiac myocytes (CM) proliferation following myocardial infarction (MI) and the remodeling of the necrotic tissue that ensues, result in non-regenerative repair. In contrast, zebrafish (ZF) can regenerate after an apical resection or cryoinjury of the heart. There is considerable interest in models where regeneration proceeds in the presence of necrotic tissue. We have developed and characterized a cautery injury model in the giant danio (GD), a species closely related to ZF, where necrotic tissue remains part of the ventricle, yet regeneration occurs. By light and transmission electron microscopy (TEM), we have documented four temporally overlapping processes: (1) a robust inflammatory response analogous to that observed in MI, (2) concomitant proliferation of epicardial cells leading to wound closure, (3) resorption of necrotic tissue and its replacement by granulation tissue, and (4) regeneration of the myocardial tissue driven by 5-EDU and [3H]thymidine incorporating CMs. In conclusion, our data suggest that the GD possesses robust repair mechanisms in the ventricle and can serve as an important model of cardiac inflammation, remodeling and regeneration. Anat Rec, 2012. © 2011 Wiley Periodicals, Inc.