Differential Expression and Regulation of Angiopoietin-2 in Mouse Uterus During Preimplantation Period

Authors

  • Bin Guo,

    1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China
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    • Bin Guo and Wei Wang contributed equally to this work

  • Wei Wang,

    1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China
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    • Bin Guo and Wei Wang contributed equally to this work

  • Shi-Jie Li,

    1. College of Life Science, Northeast Agricultural University, Harbin, People's Republic of China
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  • Yu-Shuai Han,

    1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China
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  • Lu Zhang,

    1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China
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  • Xue-Ming Zhang,

    1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China
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  • Ju-Xiong Liu,

    Corresponding author
    1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China
    • College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China
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  • Zhan-Peng Yue

    Corresponding author
    1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China
    • College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China
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Abstract

Angiogenesis is crucial to successful implantation and decidualization, however, as an important angiogenic growth factor, the effect of Ang-2 in the process of implantation and decidualization is still unknown. This study is to investigate the differential expression of Ang-2 in mouse uterus during early pregnancy and its regulation by steroid hormones using in situ hybridization and RT-PCR. There is no detectable Ang-2 mRNA signal on days 1–5 of pregnancy by in situ hybridization. On days 6–8, Ang-2 mRNA is mainly expressed in the primary decidua of mesometrial side, and the expression gradually increases. By RT-PCR, a significantly higher level of Ang-2 expression is observed on day 8 of pregnancy, although Ang-2 expression can be found through days 1–8. Similarly, Ang-2 is highly expressed in decidualized cells under artificial decidualization. In the ovariectomized mouse uterus, Ang-2 expression gradually increases after estrogen injection and with peak levels at 12 hr, while progesterone injection can cause a decline in uterine Ang-2 mRNA level, which reaches a nadir at 12 hr. These results suggest that Ang-2 may play a key role in the process of mouse decidualization. Estrogen can induce the expression of Ang-2 while progesterone can inhibit its expression in the ovariectomized mouse uterus. Anat Rec, 2012. © 2011 Wiley Periodicals, Inc.

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