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AR_22419_sm_SuppFig1.tif7206KSupplemental Figure 1: Characterization of srGAP1 and srGAP3 antibody specificity using primary culture of DRG as positive control and antibody omission and preabsorption as negative controls. The left panels show positive labeling for the mouse anti-srGAP1 antibody and the rabbit and rat antibodies against srGAP3. In the absence of the primary antibody (middle panels) or the addition of excessive immunogenic peptides (right panels) in the incubation buffer, no specific immunolabeling is found in the DRG sections. Scale bar = 100 µm.
AR_22419_sm_SuppFig2.tif2007KSupplemental Figure. 2: Expression of srGAP1 and srGAP3 in the proximal stump of the sciatic nerve. Fairly distinct srGAP1 immunofluorescence is present in the nerve trunk (A), with the labeled profiles appearing as longitudinal bundles and arranging in a similar fashion as that of S100 labeling (E). srGAP3 labeling is fairly low in the proximal nerve stump (B, D). Scale bar = 40 µm.

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