Three-Dimensional Analysis of the Islet Vasculature

Authors

  • Y. El-Gohary,

    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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    • Y. El-Gohary, S. Sims-Lucas, and N. Lath contributed equally to this article.

  • S. Sims-Lucas,

    1. Division of Nephrology, Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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    • Y. El-Gohary, S. Sims-Lucas, and N. Lath contributed equally to this article.

  • N. Lath,

    1. Department of General Surgery, Morristown Memorial Hospital, Morristown, New Jersey
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    • Y. El-Gohary, S. Sims-Lucas, and N. Lath contributed equally to this article.

  • S. Tulachan,

    1. Department of Internal Medicine, St. Elizabeth Health Center, Youngstown, Ohio
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  • P. Guo,

    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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  • X. Xiao,

    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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  • C. Welsh,

    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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  • J. Paredes,

    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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  • J. Wiersch,

    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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  • K. Prasadan,

    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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  • C. Shiota,

    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
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  • G.K. Gittes

    Corresponding author
    1. Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
    • Department of Pediatric Surgery, Children's Hospital of Pittsburgh of UPMC, Pittsburgh, PA
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Abstract

The pancreatic islets of Langerhans are highly vascularized structures scattered throughout the pancreas that contain a capillary network 5–10 times denser than that of the exocrine pancreas. A simple method for three-dimensional (3D) analysis of this intricate intraislet vasculature has been difficult because of the intrinsic opacity of the pancreas. We developed a whole-mount imaging technique that allows relatively easy visualization of the islet vasculature. In combination with confocal microscopy and the use of 3D imaging software, we were able to readily reconstruct the 3D architecture of an islet, allowing delineation of the islet volume, length of the intraislet vessels, and the number of vessel branch-points. This technique allows for straightforward 3D image analysis that may help toward understanding islet function. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.

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