Low Dose Oxidative Stress Induces Mitochondrial Damage in Hair Cells
Article first published online: 8 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
The Anatomical Record
Special Issue: The Anatomy and Biology of Hearing and Balance: Cochlear and Vestibular Implants
Volume 295, Issue 11, pages 1868–1876, November 2012
How to Cite
Baker, K. and Staecker, H. (2012), Low Dose Oxidative Stress Induces Mitochondrial Damage in Hair Cells. Anat Rec, 295: 1868–1876. doi: 10.1002/ar.22594
- Issue published online: 22 OCT 2012
- Article first published online: 8 OCT 2012
- Manuscript Received: 24 JUL 2012
- Manuscript Accepted: 24 JUL 2012
- oxidative stress;
- hair cells;
Oxidative stress has been implicated as a cause of hair cell damage after ischemia reperfusion injury, noise trauma, and ototoxic injury. Oxidative stress can induce both apoptosis or necrosis depending on the degree of exposure. To study how reactive oxygen species (ROS) interacts with hair cells, we have developed an in vitro model of oxidative stress using organ of Corti cultures exposed to physiologically relevant concentrations of hydrogen peroxide (H2O2). Treatment of organ of Corti cultures with low concentrations of H2O2 results in loss of outer hair cells in the basal turn of the explant. Higher concentrations of peroxide result in more extensive outer hair cell injury as well as loss of inner hair cells. Early outer hair cell death appears to occur though apoptosis as demonstrated by staining of activated caspase. The effect of oxidative stress on mitochondrial function is a key determinant of degree of damage. Oxidative stress results in reduction of the mitochondrial membrane potential and reduction of mitochondrial produced antioxidants. Low doses of oxidative stress induce changes in mitochondrial gene expression and induce mitochondrial DNA deletions. Recurrent oxidative stress or inhibition of mitochondrial function significantly enhanced hair cell death. This tissue culture model of oxidative hair cell injury maintains a pattern of injury similar to what is observed in vivo after oxidative injury and can be used to study the effects of ROS on hair cells over the time period of the culture. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.