MiR-16-1 Plays a Role in Reducing Migration and Invasion of Glioma Cells

Authors

  • Xiangdong Li,

    Corresponding author
    • Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, China
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    • X.L., N.L., and Y.B. contributed equally to this work.

  • Nan Ling,

    1. Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, China
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    • X.L., N.L., and Y.B. contributed equally to this work.

  • Yanyan Bai,

    1. Department of Neurology, The Affiliated Jiangyin Hospital of Southest University Medical College, Jiangyin, China
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    • X.L., N.L., and Y.B. contributed equally to this work.

  • Wanli Dong,

    1. Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, China
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  • Guo-Zhen Hui,

    1. Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, China
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  • Dinghua Liu,

    1. Department of Neurology, The Affiliated Jiangyin Hospital of Southest University Medical College, Jiangyin, China
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  • Jun Zhao,

    1. Department of Thoracic and cardiac Surgery, The First Affiliated Hospital, Soochow University, Suzhou, China
    2. Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, China
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  • Jin Hu

    Corresponding author
    1. Department of Neurosurgery, Shanghai Huashan Hospital, Fudan University, Shanghai, China
    • Department of Neurology, The Affiliated Jiangyin Hospital of Southest University Medical College, Jiangyin, China
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Correspondence to: Xiangdong Li, MD, Department of Neurosurgery, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, People's Republic of China. E-mail: xdlijia@yahoo.com.cn and Jin Hu, MD, Department of Neurosurgery, Shanghai Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, Shanghai 200040, People's Republic of China. E-mail: sososyr@hotmail.com

Abstract

MicroRNAs (miRNAs) are novel small noncoding RNA molecules that regulate gene expression at the post transcriptional level. Compelling evidence shows that there are causative links between miRNAs deregulation and cancer development and progression. This study aims to explore the functions of miR-16-1 on proliferation, apoptosis, motility, and invasion of glioma cells. Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of miR-16-1 in normal brain tissues and two glioma cell lines, including U251 and U87. CCK-8, Annexin V/FITC (fluorescein isothiocyanate), wound healing, and transwell assays were used to evaluate the functions of miR-16-1 that involves cell proliferation, apoptosis, motility, and invasion. In addition, we conducted qRT-PCR to examine mRNA expression levels of Zyxin, one of putative target genes of miR-16-1, in U251 glioma cells after transfecting with miR-16-1 mimics. As a result, miR-16-1 expression level was lower in U251 and U87 cells than normal brain tissues. After miR-16-1 was upregulated in U251 cells, cellular proliferation was notably attenuated but cell apoptosis was not significantly increased. Moreover, overexpression of miR-16-1 attenuated migration and invasion of glioma cells, and U251 cells transfected with miR-16-1 showed significantly lower endogenous mRNA levels of Zyxin than those transfected with nonspecific control miRNA or mock (P < 0.05). In summary, we demonstrated that miR-16-1 expression was markedly decreased in human glioma cell lines, and for the first time, described the roles of miR-16-1 in cellular proliferation, migration, and invasion abilities of high-invasive glioma cells, and suggested that Zyxin may be one of putative target genes of miR-16-1. Anat Rec, 296:427–432, 2012. © 2012 Wiley Periodicals, Inc.

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