Juan Yang and Li-Li Hu contributed equally to this work.
Proteomics Reveals Intersexual Differences in the Rat Brain Hippocampus
Article first published online: 5 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
The Anatomical Record
Volume 296, Issue 3, pages 462–469, March 2013
How to Cite
Yang, J., Hu, L.-L., Liu, L.-Y., Zhao, L.-Y., Hou, N., Ni, L., Li, Z.-F., Wang, A.-Y., Song, T.-S. and Huang, C. (2013), Proteomics Reveals Intersexual Differences in the Rat Brain Hippocampus. Anat Rec, 296: 462–469. doi: 10.1002/ar.22651
- Issue published online: 13 FEB 2013
- Article first published online: 5 FEB 2013
- Manuscript Accepted: 29 NOV 2012
- Manuscript Received: 21 SEP 2012
- National Natural Science Foundation of China . Grant Number: 81200845
- National Science Foundation for Postdoctoral Scientists of China . Grant Number: 20090461301
- Young Scientist Foundation from the Medical School in Xi'an Jiaotong University . Grant Number: YQN0809
- Scientific Research Support Program for New Teachers . Grant Number: 0116-081410-05
- Guang Hua Medical Innovation Research Foundation . Grant Number: 0203407
- Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) . Grant Number: 1171
- intersexual differences;
- protein expression;
It is widely accepted that intersexual differences occur in cognitive domains, e.g., in spatial learning and memory. The hippocampus plays important roles in the consolidation of information from short-term memory to long-term memory and spatial navigation. However, it still remains unknown whether the hippocampal proteomic profiling differs between males and females. In this study, we investigated the intersexual differences in protein expression of hippocampi using the two-dimensional electrophoresis analysis. In all, 33 differentially expressed proteins were characterized by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry and validated by Western-blotting analysis. In line with Western-blotting validation, the proteomic identification revealed the overexpression of glial fibrillary acidic protein in female rats' hippocampi, and the overexpression of both creatine kinase B-type and DRP-2 in male rats' hippocampi. The intersexual differences in hippocampal proteomic profiling are probably closely related to those in spatial learning and memory abilities. Anat Rec, 296:462–469, 2013. © 2013 Wiley Periodicals, Inc.