Growth-Inhibitory Activity of Melatonin on Murine Foregastric Carcinoma Cells In Vitro and the Underlying Molecular Mechanism

Authors

  • Li Xu,

    1. Department of Human Anatomy, Histology and Embryology, Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Neurobiology Research Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, People's Republic of China
    Current affiliation:
    1. Putian University, Fujian, People's Republic of China
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  • Hui Liu,

    1. Department of Human Anatomy, Histology and Embryology, Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Neurobiology Research Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, People's Republic of China
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  • Hui Zhang,

    1. Department of Human Anatomy, Histology and Embryology, Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Neurobiology Research Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, People's Republic of China
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  • Ri-Xiong Wang,

    1. Department of Human Anatomy, Histology and Embryology, Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Neurobiology Research Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, People's Republic of China
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  • Jun Song,

    1. Department of Human Anatomy, Histology and Embryology, Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Neurobiology Research Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, People's Republic of China
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  • Rui-Xiang Zhou

    Corresponding author
    • Department of Human Anatomy, Histology and Embryology, Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Neurobiology Research Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, People's Republic of China
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Correspondence to: Rui-Xiang Zhou, Department of Human Anatomy, Histology and Embryology, Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Neurobiology Research Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. Fax: +86-591-22862273. E-mail: zhourx@mail.fjmu.edu.cn

ABSTRACT

Melatonin (MLT) is an indolic hormone produced mainly by the pineal gland. Recent human and animal studies have shown that MLT exerts obvious oncostatic activity both in vitro and in vivo. The purpose of this study was to investigate the antiproliferative effect of MLT on the murine foregastric carcinoma (MFC) cell and to determine the underlying molecular mechanism. Cell viability was determined using the Cell Counting Kit-8 (CCK-8) and the results revealed that MLT exhibited a dose- and time-dependent inhibitory effect on MFC cell growth. Our studies also demonstrated upregulation of p21 and Bax and downregulation of Bcl-2 at both the mRNA and the protein levels in response to MLT treatment of MFC cells. These changes in the expression of these molecules were consistent with the results of the CCK-8. Furthermore, the mRNA and protein expression of membranous MLT receptors was also upregulated. Taken together, these results confirm the oncostatic effect of MLT in MFC cells and the expression of membranous MLT receptors is a potential approach to tumor cells in gastric cancer therapeutic treatment. Anat Rec, 296:914–920, 2013. © 2013 Wiley Periodicals, Inc.

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