Cell Proliferation Patterns in Early Zebrafish Development

Authors

  • Mario A. Mendieta-Serrano,

    1. Departamento de Fisiología Molecular y Genética del Desarrollo, Instituto de Biotecnología, Universidad Nacional Autónoma de México, C.P., México
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  • Denhi Schnabel,

    1. Departamento de Fisiología Molecular y Genética del Desarrollo, Instituto de Biotecnología, Universidad Nacional Autónoma de México, C.P., México
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  • Hilda Lomelí,

    1. Departamento de Fisiología Molecular y Genética del Desarrollo, Instituto de Biotecnología, Universidad Nacional Autónoma de México, C.P., México
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  • Enrique Salas-Vidal

    Corresponding author
    • Departamento de Fisiología Molecular y Genética del Desarrollo, Instituto de Biotecnología, Universidad Nacional Autónoma de México, C.P., México
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Correspondence to: Enrique Salas-Vidal, Departamento de Fisiología Molecular y Genética del Desarrollo, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad #2001, Colonia Chamilpa, Cuernavaca, Morelos, C.P. 62210, México. E-mail: esalas@ibt.unam.mx

ABSTRACT

Although cell proliferation is an essential cell behavior for animal development, a detailed analysis of spatial and temporal patterns of proliferation in whole embryos are still lacking for most model organisms. Zebrafish embryos are particularly suitable for this type of analysis due to their transparency and size. Therefore, the main objective of the present work was to analyze the spatial and temporal patterns of proliferation during the first day of zebrafish embryo development by indirect immunofluorescence against phosphorylated histone H3, a commonly used mitotic marker. Several interesting findings were established. First, we found that mitosis metasynchrony among blastomeres could begin at the 2- to 4-cell stage embryos. Second, mitosis synchrony was lost before the midblastula transition (MBT). Third, we observed a novel pattern of mitotic clusters that coincided in time with the mitotic pseudo “waves” described to occur before the MBT. Altogether, our findings indicate that early development is less synchronic than anticipated and that synchrony is not a requirement for proper development in zebrafish. Anat Rec, 296:759–773, 2013. © 2013 Wiley Periodicals, Inc.

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