The Superficial Glia Limitans of Mouse and Monkey Brain and Spinal Cord

Authors

  • Xiaofeng Liu,

    1. Department of Neurobiology, MOE Key Laboratory of Molecular Neurobiology, Ministry of Education, Neuroscience Research Centre of Changzheng Hospital, Second Military Medical University, Shanghai, People's Republic of China
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  • Zhaohuan Zhang,

    1. Department of Neurology and Neuroscience Research Centre of Changzheng Hospital, Second Military Medical University, Shanghai, People's Republic of China
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  • Wei Guo,

    1. Department of Neurobiology, MOE Key Laboratory of Molecular Neurobiology, Ministry of Education, Neuroscience Research Centre of Changzheng Hospital, Second Military Medical University, Shanghai, People's Republic of China
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  • Geoffrey Burnstock,

    1. Autonomic Neuroscience Centre, University College Medical School, London, United Kingdom
    2. Department of Pharmacology, Melbourne University, Victoria, Australia
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  • Cheng He,

    1. Department of Neurobiology, MOE Key Laboratory of Molecular Neurobiology, Ministry of Education, Neuroscience Research Centre of Changzheng Hospital, Second Military Medical University, Shanghai, People's Republic of China
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  • Zhenghua Xiang

    Corresponding author
    • Department of Neurobiology, MOE Key Laboratory of Molecular Neurobiology, Ministry of Education, Neuroscience Research Centre of Changzheng Hospital, Second Military Medical University, Shanghai, People's Republic of China
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  • X. Liu and Z. Zhang contributed equally to this work.

Correspondence to: Zhenghua Xiang, Department of Neurobiology, MOE Key Laboratory of Molecular Neurobiology, Ministry of Education, Neuroscience Research Centre of Changzheng Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China. Fax: +86-21-6549-2132. E-mail: zhxiang@hotmail.com

ABSTRACT

Using the double immunostaining method, the glia limitans on the surfaces of mouse and monkey brain and spinal cord were studied systematically. The results showed that the superficial glia limitans of mouse brain and spinal cord comprise a layer of surface astrocytes, while the glia limitans of monkey comprise a layer of foot-plates from marginal regions as described in histology and neuroscience textbooks. These surface astrocytes first appear at embryonic day (E)16 in spinal cord and at E17 on the ventral surface of the brain. At postnatal day (P)1, a layer of astrocytes covered the outermost regions of the spinal cord. At P10, the layer of astrocytes also covered the brain surface. The highest proliferation rates of surface astrocytes were found at E17 in the spinal cord and at E18 in the forebrain. Anat Rec, 296:995–1007, 2013. © 2013 Wiley Periodicals, Inc.

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