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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
ar22717-sup-0001-suppfig1.tif16143KSupporting Figure 1. Surface astrocytes double labeled with GFAP and GS antibodies in the horizontal section of the dorsal superficial glial limitans of adult mouse forebrain. A shows two typical surface astrocytes with GFAP-ir in the horizontal section of the dorsal superficial forebrain. B shows GS-ir astrocytes in the same field as A. C shows the merged images of A, B and counter-stained with DAPI. D shows the surface astrocytes with GFAP-ir in the horizontal section of the lateral superficial forebrain. E shows GS-ir astrocytes in the same field as D. C shows the merged images of D, E and counter-stained with DAPI. v, vessel. All scale bars = 40μm.
ar22717-sup-0002-suppfig2.tif17663KSupporting Figure 2. GFAP-immunoreactive (ir) surface astrocytes on the dorsal superficial glial limitans of adult rat, guinea pig, chicken and rabbit forebrain. A, B, C and D shows GFAP-ir surface astrocytes on dorsal superficial glial limitans of rat, guinea pig, rabbit and chicken forebrain respectively. Note that the glial limitans of these four aninal forebrains was found to be mainly composed of a layer of astroytes. All scale bars = 130μm.

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