Is Mda-7/IL-24 a Potential Target and Biomarker for Enhancing Drug Sensitivity in Human Glioma U87 Cell Line?
Article first published online: 24 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
The Anatomical Record
Volume 296, Issue 8, pages 1154–1160, August 2013
How to Cite
Wang, Q., Zhu, Y. and Yang, P. (2013), Is Mda-7/IL-24 a Potential Target and Biomarker for Enhancing Drug Sensitivity in Human Glioma U87 Cell Line?. Anat Rec, 296: 1154–1160. doi: 10.1002/ar.22723
- Issue published online: 13 JUL 2013
- Article first published online: 24 JUN 2013
- Manuscript Accepted: 16 APR 2013
- Manuscript Revised: 5 APR 2013
- Manuscript Received: 21 FEB 2013
- drug resistance reversal
Gliomas are the most common form of primary brain tumor with the highest mortality rates. Drug resistance is a major cause of treatment failure in patients with glioma. Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24) has been demonstrated to play an important role in drug resistance in human cancer cell lines. However, the reversing effect of mda-7/IL-24 on drug resistance of human glioma is not fully clear. Here, we investigated the effects of overexpression of the mda-7/IL-24 gene in human glioma. We established a cisplatin-resistant U87 glioma cell line and found that mda-7/IL-24 was highly correlated with drug resistance. Furthermore, we investigated the apoptotic rate, intracellular accumulation of Rhodamine-123, and expression of glutathione and P-glycoprotein. The over-expression of mda-7/IL-24 enhanced cisplatin cytotoxicity and reversal of drug resistance in glioma cells. The reversing effect of mda-7/IL-24 on drug resistance was induced mainly through the regulation of drug resistance-related genes and efflux drug pumps. Thus, mda-7/IL-24 can be used as a promising predictive biomarker and potential therapeutic target for chemotherapy in glioma. Anat Rec, 2013. © 2013 Wiley Periodicals, Inc.