Distribution of Transglutaminase 6 in the Central Nervous System of Adult Mice

Authors

  • Yu-Tao Liu,

    1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
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  • Bei-Sha Tang,

    1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
    2. State Key Laboratory of Medical Genetics, Changsha, China
    3. Neurodegenerative Disorders Research Center, Central South University, Changsha, China
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  • Wei Lan,

    1. Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China
    2. Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai, China
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  • Ning-Ning Song,

    1. Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China
    2. Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai, China
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  • Ying Huang,

    1. Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China
    2. Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai, China
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  • Lei Zhang,

    1. Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China
    2. Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai, China
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  • Wen-Juan Guan,

    1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
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  • Yu-Ting Shi,

    1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
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  • Lu Shen,

    1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
    2. State Key Laboratory of Medical Genetics, Changsha, China
    3. Neurodegenerative Disorders Research Center, Central South University, Changsha, China
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  • Hong Jiang,

    1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
    2. State Key Laboratory of Medical Genetics, Changsha, China
    3. Neurodegenerative Disorders Research Center, Central South University, Changsha, China
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  • Ji-Feng Guo,

    1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
    2. State Key Laboratory of Medical Genetics, Changsha, China
    3. Neurodegenerative Disorders Research Center, Central South University, Changsha, China
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  • Kun Xia,

    1. State Key Laboratory of Medical Genetics, Changsha, China
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  • Yu-Qiang Ding,

    1. Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China
    2. Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai, China
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  • Jun-Ling Wang

    Corresponding author
    1. State Key Laboratory of Medical Genetics, Changsha, China
    2. Neurodegenerative Disorders Research Center, Central South University, Changsha, China
    • Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
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Correspondence to: Dr. Junling Wang, Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan province, 410008, China. Fax: +86-(731)−84–327-332. E-mail:wjling8002@yahoo.com.cn or wjling8002@126.com

ABSTRACT

Our previous study identified a new form of spinocerebellar ataxia (SCA), in which mutations in the gene coding for transglutaminase 6 (TG6) were suggested to be causative. However, the data concerning cellular distribution of TG6 in the brain is still fragmentary. Therefore, we now report a comprehensive immunohistochemical examination of the expression profile of TG6 in adult mouse brain. TG6 was abundantly expressed in the septal region, basal ganglia, hypothalamus and brainstem. Notably, numerous TG6-positive neurons were found in the key brain regions involved in regulating locomotion activity, including the globus pallidus, subthalamic nucleus, substantia nigra, cerebellum, some precerebellar nuclei, and spinal motor neurons. Double immunostaining showed that the vast majority of TG6-positive neurons in the reticular nigra were GABAergic and those in the compact nigra were not dopaminergic. In addition, double staining for TG6 with either anti-NeuN or glial fibrillary acidic protein (GFAP) antibodies demonstrated exclusive NeuN-TG6 co-localization. This study presents a comprehensive overview of TG6 expression in the mouse brain, and provides insight for investigating the role of TG6 in the development of SCA. Anat Rec, 296:1576–1587, 2013. © 2013 Wiley Periodicals, Inc.

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